Isolation and Characterization of Sea Aster Salt-Stress Responsive Cysteine Protease Gene Obtained by a Hetero-probed Macroarray
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概要
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To isolate and analyze salt-stress inducible genes in a halophyte, sea aster (Aster tripolium L.), we screened 5760 Arabidopsis cDNA clones by macroarray procedure using 33P-labeled cDNA targets synthesized from mRNAs isolated from NaCl treated and untreated sea aster seedlings. Seventeen Arabidopsis cDNAs were hetero-hybridized to NaCl inducible sea aster genes. These cDNAs were used as probes to isolate cDNA homologs from a sea aster cDNA library. One of the obtained cDNAs shared 71% amino acids identity with Arabidopsis cysteine protease (AtCysP) and named SaCysP (sea aster CysP). Northern blot analysis revealed that mRNAs corresponding to both SaCysP and AtCysP were induced by salt and osmotic stress in leaves. On the other hand, SASR21 mRNA encoding another CysP in sea aster was irresponsive to these stress in leaves but respond in roots. SaCysP and SASR21 genes may have a tissue-specific function in stress response by modulating their expression levels.
- 日本植物細胞分子生物学会の論文
- 2004-06-01
著者
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SHIBATA Daisuke
Kazusa DNA Research Institute
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Uno Yuichi
Plant Resource Science, Graduate School of Agricultural Science, Kobe University
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Kanechi Michio
Plant Resource Science, Graduate School of Agricultural Science, Kobe University
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Inagaki Noboru
Plant Resource Science, Graduate School of Agricultural Science, Kobe University
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Inagaki Noboru
Plant Resource Science Graduate School Of Agricultural Science Kobe University
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Inagaki Noboru
Graduate School Of Science And Technology Kobe University
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Takeda M
Kobe Univ. Kobe Jpn
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Takeda Migiwa
Graduate School Of Science And Technology Kobe University
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Kuwata Chikara
Kazusa DNA Research Institute
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KANECHI Michio
Graduate School of Science and Technology, Kobe University
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UNO Yuichi
Faculty of Agriculture, Kobe University
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Kuwata Chikara
Kazusa Dna Res. Inst.
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Kanechi Michio
Plant Resource Science Graduate School Of Agricultural Science Kobe University
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Kanechi Michio
Graduate School Of Science And Technology Kobe University
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Uno Yuichi
Japan Int. Res. Cent. Agric. Sci.:fac. Agr. Kobe Univ.
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Uno Yuichi
Plant Resource Science Graduate School Of Agricultural Science Kobe University
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Uno Yuichi
Faculty Of Agriculture Kobe University
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Shibata Daisuke
Kazusa Dna Res. Inst. Chiba Jpn
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Shibata Daisuke
Kazusa Dna Res. Inst.
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