Screening for γ-aminobutyric Acid (GABA)-rich Tomato Varieties
スポンサーリンク
概要
- 論文の詳細を見る
γ-aminobutyric acid (GABA) is a four-carbon non-protein amino acid that is present in a wide variety of prokaryotic and eukaryotic organisms. Because of its antihypertensive effect on the human body, the demand for naturally occurring GABA has increased recently in the Japanese food industry. In this study, we evaluated the GABA content of tomato fruits of 61 commercial cultivars, wild species, and wild derivatives in 2005 and 2006 to screen for resources to breed a GABA-rich cultivar. GABA contents in tomato fruits greatly varied among the tested varieties and were poorly reproducible between the tested years. GABA-rich candidates selected from the screening were then subjected to salinity-stressed cultivation using the NFT system to assess their suitability for cultivation to produce GABA-rich fruit. Based on the results of two screenings and the salinity-stress cultivation test, 'DG03-9' was selected as a GABA-rich cultivar. The accumulation profiles for GABA, glutamine, and glutamic and aspartic acid during fruit development were also investigated in 'DG03-9' and 'House Momotaro' under salinity stress. The GABA content peaked at 24 days after flowering (DAF) in 'DG03-9' and 36 DAF in 'House Momotaro', and then declined during ripening. Salinity stress apparently promoted GABA accumulation during the early developing stages, but its effect on GABA decrease was different between the varieties. Although the GABA content in red mature fruits of 'DG03-9' was higher than that in fruits of 'House Momotaro' under normal and saline conditions, the maximum contents were almost the same in both cultivars. These results suggest that the lower reduction rate of GABA during the ripening stage causes high GABA accumulation in 'DG03-9' fruit. This variety will be a useful resource in the breeding of new GABA-rich cultivars. Additionally, we utilized an enzymatic assay with GABase to quantify GABA content in tomato fruit. This method will be a powerful screening tool for breeding.
- 園芸学会の論文
著者
-
EZURA Hiroshi
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
FUKUDA Naoya
Graduate School of Life and Environmental Science, University of Tsukuba
-
江面 浩
筑波大学大学院生命環境科学研究科遺伝子実験センター
-
江面 浩
筑波大学大学院生命環境科学研究科生物圏資源科学専攻
-
Saito Takeshi
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Inai Shuji
Nippon Del Monte Corporation
-
Fujii Takashi
Nippon Del Monte Corporation
-
Konishi Chiaki
Nippon Del Monte Corporation
-
江面 浩
筑波大 大学院生命環境科学研究科 遺伝子実験セ
-
Iijima Yoko
Kazusa Dna Research Institute
-
Iijima Yoko
Kazusa Dna Res. Inst. Chiba Jpn
-
Matsukura Chiaki
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Sugiyama Masaki
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Watahiki Akiko
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Ohshima Izumi
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Nishimura Shigeo
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Fukuda Naoya
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Fukuda Naoya
Graduate School Of Life And Environmental Science University Of Tsukuba
-
Ezura Hiroshi
Graduate School Of Life And Environmental Sci. Univ. Of Tsukuba
-
Ohshima Izumi
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Watahiki Akiko
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Sugiyama Masaki
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
斎藤 岳士
大阪府大院生命環境科学研究科
-
Nishimura Shigeo
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Saito Takeshi
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
江面 浩
筑波大学大学院生命環境科学研究科
-
Fujimura Tatsuhito
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Matsukura Chiaki
Graduate School Of Life And Environmental Sciences University Of Tsukuba
-
Fujimura Tatsuhito
Nippon Del Monte Corp.
-
Iijima Yoko
Kazusa Dna Res. Institutes
-
Ezura Hiroshi
Graduate School Of Life And Environment Sciences Univ. Of Tsukuba
-
Saito Takeshi
Graduate Department Of Gene Science Faculty Of Sci. Hiroshima Univ.
-
Fukuda Naoya
Graduate School of Life and Environmental Sciences, University of Tsukuba
-
Matsukura Chiaki
Graduate School of Life and Environment Sciences, University of Tsukuba
関連論文
- Metabolic alterations in organic acids and γ-aminobutyric acid in developing tomato (Solanum lycopersicum L.) fruits
- 塩ストレス,栽植密度ならびに果房直下の側枝がNFT栽培トマトの収量および糖度に及ぼす影響(栽培管理・作型)
- Research tools for functional genomics in melon (Cucumis melo L.) : Current status and prospects
- Ectopic expression of an AP3-like and a PI-like genes from 'Sekkoku' orchid (Dendrobium moniliforme) causes the homeotic conversion of sepals to petals in whorl 1 and the suppression of carpel development in whorl 4 in Arabidopsis flowers
- 体細胞変異を利用した低温肥大性メロン品種の育成
- Effects of Root-volume Restriction and Salinity on the Fruit Yield and Quality of Processing Tomato
- トマトGABA高蓄積系統・品種の選抜と塩ストレス栽培の適用
- 遺伝子組換えトマトを使った甘味誘導タンパク質ミラクリンの生産
- Screening for γ-aminobutyric Acid (GABA)-rich Tomato Varieties
- Overexpression of apple spermidine synthase 1 (MdSPDS1) leads to significant salt tolerance in tomato plants
- Molecular and genetic characterization of transgenic tomato expressing 3-hydroxy-3-methylglutaryl coenzyme A reductase
- 塩ストレス処理はトマト果実発達過程初期のデンプン合成を促進する
- メロンのエチレン受容体遺伝子を利用した組換え植物の稔性低下技術
- ウリ科作物の改良において利用されるバイオテクノロジーの現状と今後に関するアンケート調査
- DNAアレイを用いたメバロン酸経路に関連したトマト果実肥大関連因子の探索
- EMS処理によるトマト (Micro-Tom) 突然変異系統群の作出・整備の現状
- エチレン受容体遺伝子の植物分子育種への新たな利用
- Salinity Stress Affects Assimilate Metabolism at the Gene-expression Level during Fruit Development and Improves Fruit Quality in Tomato (Solanum lycopersicum L.)
- トマト品種'DG03-9'に存在するγ-アミノ酪酸(GABA)高蓄積形質の遺伝解析
- Gene dosage and genetic background affect miraculin accumulation in transgenic tomato fruits
- 赤色ならびに青色発光ダイオード下において栽培したペチュニアとシロイヌナズナの花成関連遺伝子発現に関する解析
- ナショナルバイオリソースプロジェクト「トマト」 Micro-Tom を基盤としたリソース整備事業について
- 〈原著論文〉 養液栽培の廃棄培養液処理における数種作物の無機要素吸収特性の研究
- 前歴の光質がペチュニアにおける花成反応および主茎伸長に与える影響
- 根域容量制限ならびに塩ストレス処理が加工用トマトの果実収量と品質に及ぼす影響
- 赤・青色光がペチュニアの花成に及ぼす影響
- 塩ストレス処理が養液栽培トマト果実の糖代謝酵素の活性に及ぼす影響
- 栽植密度が塩ストレス処理した養液栽培トマトの収量ならびに糖含量に及ぼす影響
- 塩ストレス処理期間が養液栽培トマトの果実肥大ならびに糖蓄積に及ぼす影響
- Aliphatic Aldehyde Reductase Activity Related to the Formation of Volatile Alcohols in Vietnamese Coriander Leaves
- Anthocyanin Compounds in Japanese Ginger (Zingiber officinale Roscoe) and Their Quantitative Characteristics
- Generation of gamma irradiation-induced mutant lines of the miniature tomato (Solanum lycopersicum L.) cultivar ′Micro-Tom′
- A tool for high-throughput prediction of molecular formulas and identification of isotopic peaks from large-scale mass spectrometry data
- Studies on Transcriptional Regulation of Endogenous Genes by ERF2 Transcription Factor in Tobacco Cells
- Metabolite Profiling of Chalcones and Flavanones in Tomato Fruit
- Application of Metabolomics to Improve Tomato Fruit Productivity and Quality
- トマトゲノム国際共同研究と今後の展望
- メロン日持ち性の分子機構解析はメロン生産・消費の回復にどのように貢献できるか? (メロンの育種と品質に関する諸問題)
- 培養液の流速と濃度が数種野菜苗の生育と無機要素吸収に及ぼす影響
- 塩ストレス処理期間および栽植密度が養液栽培トマトの果実の大きさならびに糖含量に及ぼす影響
- 塩ストレス, 栽植密度および果房直下の側枝が養液栽培トマトの収量ならびに糖含量に及ぼす影響
- Protective Roles of Bacterioruberin and Intracellular KCl in the Resistance of Halobacterium salinarium against DNA-damaging Agents
- Mutant Resources for the Miniature Tomato (Solanum lycopersicum L.) 'Micro-Tom'
- A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics
- Efficacy of a special screened greenhouse covered by duplex fine mesh in reducing maize outcrossing
- Effect of a special screened greenhouse covered by fine mesh on maize outcrossing
- Reducing gene flow from pollen dispersal of genetically modified plants in special screened greenhouses
- Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens
- Biologically Active Glycosides from Asteroidea, 41. : Isolation and Structure Determination of Glucocerebrosides from the Starfish Linckia laevigata
- Effects of Salinity on Distribution of Photosynthates and Carbohydrate Metabolism in Tomato Grown using Nutrient Film Technique
- Metabolic Alterations in Organic Acids and γ-Aminobutyric Acid in Developing Tomato (Solanum lycopersicum L.) Fruits
- Biochemical Mechanism on GABA Accumulation During Fruit Development in Tomato
- Microbial Diversity in Milled Rice as Revealed by Riosomal Intergenic Spacer Analysis
- Community Analysis of Seed-Associated Microbes in Forage Crops using Culture-Independent Methods
- Inclusion of Tomato in the Genus Solanum as "Solanum lycopersicum" is Evident from Phylogenetic Studies
- Analysis of Molecular Diversity of Bacterial Chitinase Genes in the Maize Rhizosphere Using Culture-Independent Methods
- Microbial Community Analysis in the Rhizosphere of a Transgenic Tomato that Overexpresses 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase
- Discrimination of the Commercial Seeds of Forage Crops using Ribosomal Intergenic Spacer Analysis
- Soil microbial community analysis in the environmental risk assessment of transgenic plants
- Application of Ribosomal Intergenic Spacer Analysis to the Characterization of Microbial Communities in Rhizospheric Soils of the Bt Transgenic Chrysanthemum
- Oxygen Permeability of Surface-modified Poly(dimethylsiloxane) Characterized by Scanning Electrochemical Microscopy
- 塩ストレス処理期間および栽植密度が養液栽培トマトの果実の大きさならびに糖含量に及ぼす影響
- 塩ストレス処理および果房直下の側枝が養液栽培トマトの果実糖度と光合成産物転流に及ぼす影響
- ナショナルバイオリソースプロジェクト・トマト(NBRPトマト) : 研究を支えるバイオリソースの整備
- A simple method for purifying undenatured miraculin from transgenic tomato fruit
- Comparative and Stability Analyses of 9- and 13-Oxo-octadecadienoic Acids in Various Species of Tomato
- Aliphatic Aldehyde Reductase Activity Related to the Formation of Volatile Alcohols in Vietnamese Coriander Leaves
- Genetic diversity of radish (Raphanus sativus) germplasms and relationships among worldwide accessions analyzed with AFLP markers
- Wireless-Caused Representative Selection Fluctuation Problem in Wireless Multicast Congestion Control(Mobile Multimedia Communications)
- Overexpression of apple spermidine synthase 1 (MdSPDS1) leads to significant salt tolerance in tomato plants
- Efficient plant regeneration from protoplasts of eggplant rootstock cultivar and its wild relatives
- Tomato is a Next-generation Model Plant for Research and Development
- Gene Expression of ADP-glucose Pyrophosphorylase and Starch Contents in Rice Cultured Cells are Cooperatively Regulated by Sucrose and ABA
- Comparative and Stability Analyses of 9- and 13-Oxo-octadecadienoic Acids in Various Species of Tomato
- A simple method for purifying undenatured miraculin from transgenic tomato fruit
- TOMATOMA : A Novel Tomato Mutant Database Distributing Micro-Tom Mutant Collections
- Red Pigment of Deinococcus radiodurans
- Cultivation under salt stress increases the concentration of recombinant miraculin in transgenic tomato fruit, resulting in an increase in purification efficiency
- Radiation Protective Effect of Carotenoids
- シンポIIはじめに
- Tomato TILLING Technology: Development of a Reverse Genetics Tool for the Efficient Isolation of Mutants from Micro-Tom Mutant Libraries
- An investigation of differences in fruit yield and components contributing to increased fruit yield in Japanese and Dutch tomato cultivars
- Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life
- Low sodium chloride priming increases seedling vigor and stress tolerance to Ralstonia solanacearum in tomato
- Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca^ utilization and promotes hypersensitivity to Na^+ and K^+ stresses
- Isolation and characterisation of the ADP-glucose pyrophosphorylase small subunit gene (AgpS1) promoter in tomato (Solanum lycopersicum L.)
- Long-Chain Free Fatty Acid Profiling Analysis by Liquid Chromatography–Mass Spectrometry in Mouse Treated with Peroxisome Proliferator-Activated Receptor α Agonist
- Iron and Ferritin Deficiencies in the Cerebral Cortex of Senescence-accelerated Mouse Prone 10
- Construction of a chromosome-assigned, sequence-tagged linkage map for the radish, Raphanus sativus L. and QTL analysis of morphological traits
- エピミュータジェネシスと次世代育種への展開
- Suppression of γ-Aminobutyric Acid (GABA) Transaminases Induces Prominent GABA Accumulation, Dwarfism and Infertility in the Tomato (Solanum lycopersicum L. )