Protective Roles of Bacterioruberin and Intracellular KCl in the Resistance of Halobacterium salinarium against DNA-damaging Agents
スポンサーリンク
概要
- 論文の詳細を見る
Halobacterium salinarium, a member of the extremely halophilic archaebacteria, contains a C_50 -carotenoid namely bacterioruberin. We have previously reported the high resistance of this organism against the lethal actions of DNA-damaging agents including ionizing radiation and ultraviolet light (UV). In this study, we have examined whether bacterioruberin and the highly concentrated salts in this bacterium play protective roles against the lethal actions of ionizing radiation, UV, hydrogen peroxide, and mitomycin-C (MMC). The colourless mutant of H. salinarium deficient in bacterioruberin was more sensitive than the red-pigmented wild-type to all tested DNA-damaging agents except MMC. Circular dichroism (CD) spectra of H. salinarium chromosomal DNA at various concentrations of KCl (0-3.5 M) were similar to that of B-DNA, indicating that no conformational changes occurred as a result of high salt concentrations. However, DNA strand-breaks induced by ionizing radiation were significantly reduced by the presence of either bacterioruberin or concentrated KCl, presumably due to scavenging of free radicals. These results suggest that bacterioruberin and intracellular KCl of H. salinarium protect this organism against the lethal effects of oxidative DNA-damaging agents.
- 日本放射線影響学会の論文
著者
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山本 修
Department Of Applied Chemistry Kanagawa Institute Of Technology
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Saito Takeshi
Graduate School of Life and Environmental Sciences, University of Tsukuba
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SAITO Takuya
Research Institute for Information Science and Education, Hiroshima University
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IDE Hiroshi
Graduate Dept. of Gene Sci., Fac. of Sci., Hiroshima Univ.
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Gekko Kunihiko
Graduate School of Science, Hiroshima University
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Ide H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Gekko Kunihiko
Graduate Department Of Gene Science Faculty Of Science Hiroshima University
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OHYAMA Yoshihiko
Graduate Department of Gene Science, Faculty of Science, Hiroshima University
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TERATO Hiroaki
Graduate Department of Gene Science, Faculty of Science, Hiroshima University
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Ide Hiroshi
Dept. Math. Life Sci. Grad. Sch. Sci. Hiroshima Univ.
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Yamamoto Osamu
Department Of Clinical Radiology Faculty Of Health Science Hiroshima International University
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ASGARANI EZAT
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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SAITO TAKESHI
Radioisotope Center, Hiroshima University
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SHAHMOHAMMADI HAMID
Graduate Department of Gene Science, Faculty of Science, Hiroshima University
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ASGARANI EZAT
Graduate Department of Gene Science, Faculty of Science, Hiroshima University
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YAMAMOTO OSAMU
Graduate Department of Gene Science, Faculty of Science, Hiroshima University
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Saito Takeshi
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Asgarani Ezat
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Saito T
Research Institute For Information Science And Education Hiroshima University
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Shahmohammadi Hamid
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Ide Hiroshi
Graduate Department Of Gene Sci. Faculty Of Sci. Hiroshima Univ.
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Yamamoto Osamu
Graduate Department Of Gene Science Faculty Of Sci. Hiroshima Univ.
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Saito Takeshi
Graduate Department Of Gene Science Faculty Of Sci. Hiroshima Univ.
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SAITO Takeshi
Department of Physics, Kyoto Prefectural University of Medicine
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IDE Hiroshi
Graduate Department of Gene Sci. , Faculty of Sci. , Hiroshima Univ.
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