Generation of gamma irradiation-induced mutant lines of the miniature tomato (Solanum lycopersicum L.) cultivar ′Micro-Tom′
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概要
- 論文の詳細を見る
One direct way to identify a gene and its function is a forward genetic approach based on mutation analyses. To accumulate genetic resources for breeding and functional genomics in the tomato, we generated 6,347 lines of an M2 population with 300 Gy of gamma-ray irradiation in the inbred miniature dwarf variety ‘Micro-Tom.’ In total, 6,301 M2 lines were screened based on morphological alteration and brix-aberration, and 237 lines were selected as mutant candidates at the first screening. Subsequent screening of the self-fertilized M3 and M4 progeny yielded 24 lines of morphological mutants and 11 lines of aberrant brix mutants. Segregation data suggested that most of the mutant lines had single recessive mutations, with the exception of two lines. The chlorophyll mutation ratio in germinated M2 seedlings was 0.37% and the actual mutant frequency was 0.5%. The selected mutant lines exhibited a wide range of mutations, including whole plant properties with a severe phenotype, which allowed for more efficient screening of knockout mutants. Three characterized mutants, pale leaf, pink, and short root are also described.
- 日本植物細胞分子生物学会の論文
- 2007-03-01
著者
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Saito Takeshi
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Yin Yong-gen
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Matsukura Chiaki
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Nishimura Shigeo
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Ban Yusuke
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Ban Yusuke
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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YAMAGUCHI Isao
Graduate School of Life and Environmental Sciences, University of Tsukuba
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INAMURA Mami
Graduate School of Life and Environmental Sciences, University of Tsukuba
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KOBAYASHI Yoshie
Graduate School of Life and Environmental Sciences, University of Tsukuba
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KUWATA Chikara
Crop Breeding Institute, Chiba Prefectural Agriculture Research Center
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IMANISHI Shunsuke
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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Inamura Mami
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Kuwata Chikara
Crop Breeding Institute Chiba Prefectural Agriculture Research Center
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斎藤 岳士
大阪府大院生命環境科学研究科
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Nishimura Shigeo
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Imanishi Shunsuke
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Imanishi Shunsuke
National Institute Of Vegetable And Tea Science Naro
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Saito Takeshi
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Matsukura Chiaki
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Kobayashi Yoshie
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Kobayashi Yoshie
Graduate School Of Humanities And Sciences Ochanomizu University
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Yamaguchi Isao
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Imanishi Shunsuke
National Inst. Of Vegetable And Tea Sci. National Agriculture And Food Res. Organization
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Saito Takeshi
Graduate Department Of Gene Science Faculty Of Sci. Hiroshima Univ.
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伴 雄介
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Matsukura Chiaki
Graduate School of Life and Environment Sciences, University of Tsukuba
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Yin Yong-Gen
Graduate School of Life and Environment Sciences, University of Tsukuba
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