Analysis of Molecular Diversity of Bacterial Chitinase Genes in the Maize Rhizosphere Using Culture-Independent Methods
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概要
- 論文の詳細を見る
DNA fragments of bacterial chitinase genes were successfully amplified from DNA extracts of maize rhizosphere and bulk soil. The molecular diversity of the bacterial chitinase genes in these soil samples was then evaluated by employing terminal-restriction fragment length polymorphism and sequencing analyses. Our results suggest the presence of novel groups of bacterial chitinase in both bulk and rhizosphere soils. Significant differences were observed, however, between the amplified chitinase genes from rhizosphere and bulk soils, and dominant clones obtained from the maize rhizosphere showed a high degree of similarity to the chitinases of Streptomyces avermitilis and close relatives there of. The current findings thus indicate the usefulness of cultureindependent methods in the assessment of molecular diversity among the chitinolytic bacterial community in soils.
- 日本微生物生態学会の論文
著者
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MINAMISAWA KIWAMU
Graduate School of Life Sciences, Tohoku University
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Ezura Hiroshi
Gene Research Center University Of Tsukuba
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Ezura Hiroshi
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Ikeda Seishi
Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba
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Ikeda Soichiro
Aspex Division Asahi Glass Co. Ltd.
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MIYASHITA Kiyotaka
National Institute for Agro-Environmental Sciences
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YTOW NOZOMI
Gene Research Center, University of Tsukuba
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Ikeda Seishi
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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FUJIMURA TATSUHITO
Gene Research Center, University of Tsukuba
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Fujimura Tatsuhito
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Minamisawa Kiwamu
Graduate School Of Life Sciences Tohoku Univ.
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Ytow Nozomi
Gene Research Center University Of Tsukuba
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Miyashita Kiyotaka
National Inst. For Agro-environmental Sciences
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Fujimura Tatsuhito
Nippon Del Monte Corp.
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Ytow N
Gene Research Center University Of Tsukuba
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Ikeda Seishi
Hokkaido Agricultural Research Center National Agriculture And Food Research Organization
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Minamisawa Kiwamu
Graduate School of Life Science, Tohoku University
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