A simple method for purifying undenatured miraculin from transgenic tomato fruit
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概要
- 論文の詳細を見る
The sweet taste-modifying protein miraculin has been successfully expressed in the tomato, a high-yield commercial plant (Sun et al. 2007). Previously proposed methods of purifying untagged miraculin from transgenic tomato fruit are laborious and time consuming and do not allow the separation of undenatured and denatured miraculin. Therefore, it is necessary to develop an efficient method of purifying undenatured miraculin from transgenic tomato fruit. In this study, we have demonstrated that the combined use of nickel-immobilized affinity chromatography and ion-exchange chromatography readily provides highly purified undenatured miraculin, as demonstrated by the observation of both a single peak from reverse phase high performance liquid chromatography and a single band from SDS-PAGE.
- 日本植物細胞分子生物学会の論文
- 2011-06-25
著者
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EZURA Hiroshi
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Ezura Hiroshi
Graduate School Of Life And Environmental Sci. Univ. Of Tsukuba
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Yoshida Shigeki
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Hiwasa Tanase
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Duhita Narendra
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Hiwasa-Tanase Kyoko
Graduate School of Life and Environmental Sciences, University of Tsukuba
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HIWASA TANASE
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Hiwasa Tanase
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Yoshida Shigeki
Graduate School Of Life And Environmental Sciences Univ. Of Tsukuba
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Duhita Narendra
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Ezura Hiroshi
Graduate School Of Life And Environment Sciences Univ. Of Tsukuba
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Yoshida Shigeki
Graduate School Of Life And Environmental Sciences Tsukuba University
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