Cloning, Expression, and Transglycosylation Reaction of Paenibacillus sp. Strain W-61 Xylanase 1
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概要
- 論文の詳細を見る
A xylanase gene, xyn1, which encodes Paenibacillus sp. strain W-61 xylanase 1 (Xyn1), was cloned in Escherichia coli. xyn1 encodes 211 amino acid residues, including 28 amino acid residues of a signal peptide. The deduced amino acid sequence of the mature Xyn1 showed 95.7%, 84.0%, and 83.7% identity to family 11 xylanases of Aeromonas caviae ME-1, Paenibacillus sp., and Bacillus stearothermophilus respectively. The physico-chemical properties of recombinant Xyn1 were very similar to those of intact Xyn1, except for the molecular mass. The pattern of xylooligosaccharides generated by rXyn1 was investigated by fluorophore-assisted carbohydrate electrophoresis (FACE). The degradation rate of xylohexaose by rXyn1 increased markedly as compared with that of xylopentaose. Xylohexaose had a single preferential point of cleavage by rXyn1. On the basis of the pattern of action of xylooligosaccharides, the number of subsites was estimated to be six. The catalytic site was located between the third and the fourth subsites from non-reducing end.
- 社団法人 日本農芸化学会の論文
著者
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金子 淳
東北大学大学院農学研究科応用微生物学分野
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Kaneko Jun
Tohoku Univ. Sendai Jpn
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WATANABE Seiji
Department of Dermatology, Kanazawa Medical University
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金子 淳
東北大農化
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Kaneko Jun
Department Of Applied Microbiology Graduate School Of Agricultural Science Tohoku University
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KAMIO Yoshiyuki
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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Yoshida Shigeki
Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Viet Dung
Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University
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