Molecular Cloning and Sequence Analysis of the Gene Encoding the H_2O-forming NADH Oxidase from Streptococcus mutans
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概要
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To discover the molecular properties of two distinct NADH oxidases, corresponding to H_2O_2-forming oxidase (NOX-1) and H_2O-forming oxidase (NOX-2) induced in Streptococcus mutans, for the first step we had cloned and sequenced the nox-1 gene encoding NOX-1. In this paper, a nox-2 gene encoding NOX-2 from S. mutans was cloned, and the nucleotides sequenced. The nox-2 gene comprises 1371 base-pairs, encoding a polypeptide of 457 amino acid residues. The deduced relative molecular mass (AL = 49919)agreed with the previous value obtained from the purified NOX-2 protein. The nox-2 gene was expressed in Escherichia coli using its own promoter. Alignment of the NOX-2 protein sequence with that of the NOX-1 showed that the proteins do not significantly resemble each other. Comparisons with the NADH oxidase from Streptococcus faecalis 10C1 yield identities of 41%. The redox-active cysteine in the enzyme from S.faecalis was found to correspond to Cys 44 in the NOX-2.
- 社団法人日本農芸化学会の論文
- 1996-01-23
著者
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SHIMADA Masayuki
Department of Chemistry, Faculty of Science, Kagoshima University
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YAMAMOTO Yoshikazu
Research Center, Nippon Paint Co., Ltd.
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MATSUMOTO Junichi
Research Laboratories, Dainippon Pharmaceutical Co., Ltd.
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KAMIO Yoshiyuki
Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University
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HIGUCHI Masako
Research Center, Nippon Paint Co., Ltd.
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Higuchi M
Tohoku Univ. Sendai Jpn
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Higuchi Masako
Research Center Nippon Paint Co. Ltd.
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SHIMADA Mamoru
Research Center, Nippon Paint Co., Ltd.
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Kamio Yoshiyuki
Department Of Agricultural Chemistry Tohoku University
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Matsumoto J
Kyushu Univ. Fukuoka Jpn
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Shimada M
Department Of Chemistry Faculty Of Science Kagoshima University
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Yamamoto Y
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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Yamamoto Y
Faculty Of Bio-resource Sciences Akita Prefectural University
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Matsumoto Junichi
Research Laboratories Dainippon Pharmaceutical Co. Ltd.
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Matsumoto Junichi
Research Center, Nippon Paint Co., Ltd.
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YAMAMOTO Yoshikazu
Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University
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MATSUMOTO Junichi
Research Center for Ubiquitous MEMS and Micro Engineering, National Institute of Advanced Industrial Science and Technology (AIST)
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