Molecular Cloning and Nucleotide Sequence of the Pectate Lyase Gene from Pseudomonas marginalis N6301

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概要

• 論文の詳細を見る

• Pectate lyase was purified approximately 29-fold to electrophoretic homogeneity from Pseudomonas marginalis N6301. A pectate lyase (PL; EC4.2.2.2) gene of the strain was cloned and expressed in Escherichia coli. The nucleotides of the PL gene (pel) were sequenced. An open reading frame that encodes a polypeptide (molecular weight: 40,812) composed of 380 amino acids including a 29 amino acid signal peptide was assigned. The structural gene of pel consisted of 1140 base pairs. The nucleotide sequence of the 5'-flanking region of pel showed a consensus sequence of the promoter region of the pectin lyase gene (pnl) in P. marginalis N6301, a Pribnow box, and a ribosome binding site as found in E. coli.

• 社団法人日本農芸化学会の論文
• 1993-06-23

著者

• IZAKI Kazuo

  Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University

• KAMIO Yoshiyuki

  Department of Applied Microbiology, Graduate School of Agricultural Science, Tohoku University

• NIKAIDOU Naoki

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• Izaki K

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Izaki Kazuo

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Nikaidou N

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Nikaidou Naoki

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Nikaidou Naoki

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Kamio Yoshiyuki

  Department Of Agricultural Chemistry Tohoku University

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