DNA Structure of Pectate Lyase I Gene Cloned from Erwinia carotovora(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The pectate lyase I (PAL I) gene was cloned from Erwinia carotovora Er. The cloned DNA, 5.1 kb, seemed to contain the region necessary for the regulation of pectin lyase formation, as PAL I was induced by pectin in E. carotovora Er 18 containing the cloned PAL I gene. PAL I had a signal peptide of 22 amino acids which seemed to be cleaved between Gly and Ala. PAL I had a M.W. of 37,587 composed of 345 amino acids. The presence of promoter and SD sequences was suggested upstream of the translational start codon of the PAL I gene. A palindromic sequence, which might be important for the regulation of PAL I gene expression, was detected downstream from the putative promoter. Another open reading frame highly homologous to the PAL I gene was detected downstream from the PAL I gene and designated the PAL X gene. This gene had 81% homology in 606 bp from the translational start codon with the PAL I gene.
- 社団法人日本農芸化学会の論文
- 1988-02-23
著者
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KOBAYASHI Ryoko
Department of Physics, Saga University
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IZAKI Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Kobayashi Ryoko
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:novo Industri Japan Lt
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Izaki Kazuo
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Ito Kazutoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University:(present Office)plant
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Ito Kazutoshi
Department Of Agricultrual Chemistry Faculty Of Agriculture Tohoku University
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NIKAIDO Naoki
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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Nikaido Naoki
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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