Some Properties of a New Amphiphilic Compound Designated as Material M from Selenomonas ruminantium(Microbiology & Fermentation Industry)

概要

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A compound containing fatty acids was detected in the residual fraction of Selenomonas ruminantium cells from which lipopolysaccharide and phospholipid had been extracted. This compound, designated as material M, showed a different mobility from those of lipopolysaccharide and phospholipid on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Since 2-^3H-glycerol was not significantly incorporated into this material and protease treatment did not affect its mobility on electrophoresis, it is not likely to be a lipoprotein-like compound. Material M was mainly localized in the inner membrane of this bacterium. Material M was purified from the phenol layer which formed on the treatment of phospholipid-deprived cells with phenol-water. The purified material M was estimated to be approximately 95% pure. The major components of the purified M, glucosamine and phosphate were detected in a molar ratio of 2:1. The fatty acid content of material M was only one-tenth that of glucosamine on a molar basis. The fatty acid composition of material M was different from those of lipopolysaccharide and phospholipid. Β-Hydroxy fatty acids, which are consistently present in lipopolysaccharide, were not detected in material M.
社団法人日本農芸化学会の論文
1987-04-23