Trigger of Damage by Oxygen Deficiency to the Acid Production System during Submerged Acetic Fermentation with Acetobacter aceti

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In order to clarify the mechanism of the phenomenon that acid productivity of Acetobacter aceti is almost completely damaged by oxygen deficiency for a short time during submerged fermentation accumulating a total acid concentration of 6% in the medium, the respiratory activity and the activities of enzymes involved in acid production of the damaged cells and the stabilities of enzymes extracted from the non-damaged cells were investigated.Respiratory activities for ethanol and acetaldehyde, and alcohol and aldehyde dehydrogenase activities, of the damaged cells decreased 20,0,20 and 50% compared to the controls, respectively.Alcohol dehydrogenase activity in the crude enzyme preparation solubilized with Triton X-100 from the membrane fraction was highly stable in culture broth containing a total acid concentration of 4.0% or less, but became quickly unstable in culture broth containing a total acid concentration exceeding 4.0%. The purified enzyme was very unstable even in culture broth of 2% total acid concentration. It was suggested from this fact that the alcohol dehydrogenase had a membrane component contributing to stability of itself. These phenomena were not observed with respect to aldehyde dehydrogenase in the membrane-solubilized preparation and the purified enzyme. Low stabilities of these enzymes in ethanol were also observed in the presence of acid of a concentration of 4% and more, compared with those in the cases of concentrations of 3% and less.
公益社団法人日本生物工学会の論文
1983-02-25

Trigger of Damage by Oxygen Deficiency to the Acid Production System during Submerged Acetic Fermentation with Acetobacter aceti

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