DNA as a Flocculation Factor in Pseudomonas sp.
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概要
- 論文の詳細を見る
A component responsible for flocculation was extracted from Pseudomonas strain C-120 by treating the cells with 3 M guanidine hydrochloride. The guanidine hydrochloride-extracted cells were reflocculated, not only with the guanidine hydrochloride extract but with DNA prepared from various bacteria. The reconstituted flocs were deflocculated by deoxyribonuclease or guanidine hydrochloride which indicated that the reconstituted flocs closely resembled natural flocs. In reconstitution experiments using Escherichia coli DNA at different molecular weights, it was found that DNA with a molecular weight higher than about 6 x 106 was required to flocculate the guanidine hydrochloride-extracted cells. Heat-denatured DNA did not flocculate the guanidine hydrochloride-extracted cells. DNA with a high molecular weight was detected in the guanidine hydrochloride extract. It was concluded that the component involved in flocculation of this organism was highly polymerized double stranded DNA.
- 社団法人 日本農芸化学会の論文
著者
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Takahashi Hajime
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku Uleiversity
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SAKKA Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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