Production of Molecular Hydrogen in Outdoor Batch Cultures of Rhodopseudomonas sphaeroides

概要

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Outdoor batch cultures of Rhodopseudomonas sphaeroides B5 were carried out from April 20 to May 15, 1981. The species was isolated in the Bangkok area. One culture (culture A) was placed vertically to the ground and the other (culture B) was inclined at 30° to receive more sunlight. In each culture, 33 liters of culture medium was used which contained 53mM DL-lactate and 5 mM L-glutamate, respectively, as electron donor and nitrogen source. During the cultural period of 25 days, total hydrogen production was 154.7 liters in culture A and 177.2 liters in culture B. These values corresponded to 69.1% and 78.0% of the theoretical value (6 mol of hydrogen formed per mol of lactate), respectively. The maximum rates of hydrogen production per hour were 2.85 liters in culture A and 4.11 liters in culture B. These results indicated that illumination was the limiting factor in photoproduction of hydrogen under these conditions. The results also suggested the importance of the culture medium temperature in hydrogen production.
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