Purification and Characterization of Xylanase A from CIostridium stercorarium F-9 and a Recombinant Escherichia coli
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概要
- 論文の詳細を見る
Xylanase A encoded by the C1ostridium stercorarium F-9 xynA gene was puritied to homogeneity from a recombinant clone of Escherichia coli. The N-terminal amino acid sequence and molecular weight (54, 000) estimated by SDS-PAGE of the purified enzyme were consistent with those deduced from the nucleotide sequence [Biosci. Biotech. Biochem. , 57, 273-277 (1993)]. A xylanase was also purified to homogeneity from a culture supernatant of C. stercorarium F-9. Its N-terminal amino acid sequence, molecular weight, and enzymatic properties were quite in agreement with those of the recombinant enzyme, indicating that the xynA gene was predominantly expressed as a xylanase gene in C. stercorarium F-9. The purified enzyme hydrolyzed xylotriose to yield xylobiose and xylose while it was less active toward xylobiose. It was optimally active at 75℃ and pH 7. 0. K_m and V_<max> were estimated to be 1. 9mg/ml and 2. 8μmol of xylose equivalent/min/μg for oat spelt xylan, respectively.
- 社団法人日本農芸化学会の論文
- 1994-08-23
著者
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Kojima Yuzo
Medical Enzyme Division, Amano Enzyme Co., Ltd.
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Kojima Yuzo
Department Of Bioscience Faculty Of Bioresources Center For Molecular Biology And Genetics Mie Unive
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Ohmiya Kunio
Department Of Food Science And Technology Faculty Of Agriculture Nagoya University
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Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
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Sakka Kazuo
Department of Bioscience, Faculty of Bioresources, Mie University
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Shimada Kyo
Department of Bioscience, Faculty of Bioresources, Mie University
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Shimada Kyo
Department Of Bioscience Faculty Of Bioresources Mie University
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Kojima Y
Medical Enzyme Division Amano Enzyme Co. Ltd.
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Sakka Kazuo
Department Of Bioscience Faculty Of Bioresources Mie University
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Ohmiya Kunio
Department Of Bioresources School Of Bioresources Mie University
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Kondo T
Tokyo Univ. Agriculture And Technol. Tokyo Jpn
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Karita Shuichi
Department Of Sustainable Resource Science Faculty Of Bioresources Mie University
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Kondo Tatsuki
Department of Bioscience, Faculty of Bioresources Center for Molecular Biology and Genetics, Mie Uni
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SAKKA Kazuo
Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku University
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