Analysis of a Clostridium josui Cellulase Gene Cluster Containing the man5A Gene and Characterization of Recombinant Man5A
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概要
- 論文の詳細を見る
A cellulase gene cluster of Clostridium josui was sequenced, and was found to encode 11 proteins responsible for cellulosome (cellulolytic complex) formation, viz., cipA, cel48A, cel8A, cel9A, cel9B, orfX, cel9C, cel9D, man5A, cel9E, and cel5B, in order from the upstream side. All the predicted enzymes had a dockerin module, suggesting that these proteins are members of the C. josui cellulosome. Among these genes, the man5A gene encoding β-mannanase was expressed in Escherichia coli and the recombinant enzyme (rMan5A) was characterized. rMan5A showed strong activity toward carob galactomannan and low activity toward guar gum, suggesting that it prefers non-galactosylated mannan to galactomannan. This enzyme hydrolyzed ivory nut mannan to produce mainly mannotriose and larger mannooligosaccharides, and was not active toward mannotriose. An antiserum raised against the recombinant enzyme detected Man5A in the culture supernatants of C. josui, which was grown on either ball-milled cellulose or glucose as a carbon source.
- 社団法人 日本農芸化学会の論文
- 2010-10-23
著者
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Goto Machio
Hitachi Instrument Engineering Co. Ltd.
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KARITA Shuichi
Graduate School of Bioresources, Mie University
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Kajino T
Special Research Laboratory Ii Toyota Central R&d Laboratories Inc.
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Goto M
Mie Univ. Tsu Jpn
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Kimura Tetsuya
National Center For Seeds And Seedlings
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Sakka K
Graduate School Of Bioresources Mie Univ.
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Shima M
Department Of Pediatrics Nara Medical University
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Sakka Kazuo
Graduate School Of Bioresources Mie Univ.
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Kimura Tetsuya
Department Of Science Kobe University
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Kimura Tetsuya
Graduate School Of Human & Environmental Studies Kyoto University
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Sakka Makiko
Graduate School Of Bioresources Mie Univ.
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Shibata M
Univ. Tokyo Tokyo Jpn
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Karita S
Graduate School Of Regional Innovation Studies Mie Univ.
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Karita Shuichi
Graduate School Of Bioresources Mie Univ
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Kimura Tetsuya
Graduate School Of Bioresources Mie University
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GOTO Masayuki
Graduate School of Bioresources, Mie University
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FUJINO Tsuchiyoshi
Graduate School of Bioresources, Mie University
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FUJINO Emi
Graduate School of Bioresources, Mie University
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Fujino Emi
Graduate School Of Bioresources Mie University
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Kitahashi T
Brain-operative Expression Laboratory Brain Science Institute
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SHIBATA Masaru
Department of Earth and Space Science, Faculty of ScienceOsaka University
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SHIBATA Masaru
Department of Earth and Space Science Faculty of Science, Osaka University
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SHIBATA Masaru
Department of Earth and Space Science, Faculty of Science Osaka University
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SHIBATA Masaru
Graduate School of Arts and Sciences, University of Tokyo
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SIBATA Masaru
Department of Physics, University of Illinois:Department of Earth and Space Science, Graduate School of Science, Osaha University
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SHIBATA Masaru
Department of Earth and Space Science, Osaka University
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