Enzymatic Synthesis of α-Linked Galactooligosaccharides Using the Reverse Reaction of a Cell-bound α-Galactosidase from Candida guilliermondii H-404

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α-Linked galactooligosaccharides (α-GOS A from galactose, and α-GOS B from lactose hydrolyzates)were synthesized using the reverse reaction of α-galactosidase from Candida guilliermondii H-404. The α-GOS A and B were isolated and their structures were identified by methylation analysis. The main product of the disaccharides in α-GOS A and α-GOS B was the (1, 6)-isomer. The remaining disaccharides consisted of (1, 3)-, (1, 2)-, and (1, 1)-isomers. Conditions for synthesis of α-GOS B from lactose hydrolyzates were examined. The yield of α-GOS B was approximately 20% when the mixture of heat-treated cells containing α-galactosidase (60 U/g galactose) and 85% lactose hydrolyzates was incubated for 90h at pH 4.5 and 50℃. The α-GOS A and B were available as the donor substrates in transgalactosylation of α-galactosidase in the same manner as melibiose.
社団法人日本農芸化学会の論文
1995-02-23