Enzymatic Synthesis of N-Acetylglucosaminyl-cyclodextrin by the Reverse Reaction of N-Acetylhexosaminidase from Jack Bean

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概要

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• Novel heterobranched cyclodextrins (CDs), N-acetyl-glucosaminyl-cyclodextrins (GlcNAc-CD), were synthesized from a mixture of GlcNAc and α, β, or γCD by the reverse reaction of N-acetylhexosaminidase from jack bean. Optimum pH and temperature for the production of GlcNAc-αCD by N-acetylhexosaminidase were pH 4.9 and 50-70℃, respectively. The maximum yield of GlcNAc-αCD was 17.5% (mol/mol) at the concentration of 1 MGlcNAc and 0.25M αCD. The reverse reaction product, GlcNAc-αCD, was separated into two peaks by HPLC analysis on the ODS column. Their structures were identified as 6-O-β-D-N-acetylglucosaminyl-αCD and 2-O-β-D-N-acetylglucosaminyl-αCD by FAB-MS and NMR spectroscopies. N-Acetylhexosaminidase from jack bean also synthesized N-acetylgalactosaminyl-αCD from N-acetylgalactosamine and αCD.

• 社団法人日本農芸化学会の論文
• 1999-10-23

著者

• Hashimoto H

  Bio Research Corporation Of Yokohama

• HARA Kozo

  Bio Research Corporation of Yokohama

• Hara Koji

  Bio Research Corporation Of Yokohama

• TANIMOTO Toshiko

  School of Pharmaceutical Sciences Mukogawa Women's University

• Fujita K

  Kyoto Prefectural Univ. Kyoto Jpn

• Fujita Koki

  Bio Research Corporation Of Yokohama

• KOIZUMI Kyoko

  School of Pharmaceutical Sciences, Mukogawa Women's University

• HASHIMOTO Hitoshi

  Bio Research Corporation of Yokohama

• HAMAYASU KENICHI

  Bio Research Corporation of Yokohama

• Koizumi K

  School Of Pharmaceutical Sciences Mukogawa Women's University

• Koizumi Kyoko

  School Of Pharmaceutical Sciences Mukogawa Women's University

• Hashimoto H

  Materials Research Institute For Sustainable Development National Institute Of Advanced Industrial S

• Hashimoto Hitoshi

  Res. Inst. For Sustainable Materials National Inst. Of Advanced Industrial Sci. And Technol.

• KITAHATA Sumio

  Osaka Municipal Technical Research Institute

• NAKANO HIROFUMI

  Osaka Municipal Technical Research Institute, and Yashiro Co, Ltd.,

• Kitahata S

  Department Of Bioscience And Biotecnology Shinshu University

• Nakano H

  Department Of Biochemistry Osaka Municipal Technical Research Institute

• Furuichi K

  Bio Research Corporation Of Yokohama

• Tanimoto T

  School Of Pharmaceutical Sciences Mukogawa Women's University

• Tanimoto Toshiko

  School Of Pharmaceutical Science Mukogawa Women's University

• Nakano H

  Kyoto Prefectural Univ. Kyoto Jpn

• Nakano Hirofumi

  Osaka Municipal Technical Research Institute

• Hashimoto H

  Department Of Biotechnology The University Of Tokyo

• Nakano Hirofumi

  Department Of Chemistry Faculty Of Science Osaka University

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