Effects of Temperature on 6-O-α-Maltosyl Cyclodextrin Production from α-Maltosylfluoride and Cyclodextrins(Biological Chemistry)

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The effects of temperature on 6-O-α-maltosyl cyclodextrins (G_2-CDs) production from α-maltosylfluoride (α-G_2F) and cyclodextrins (CDs) by the transfer action of debranching enzymes such as pullulanase and isoamylase were studied. The amounts of 6-O-α-maltosyl α-cyclodextrin (G_2-α-CD) production by purified pullulanse from Aerobacter aerogenes (A-pullulanase) and from Bacillus acidopullulyticus (B-pullulanase) increased with a rise in temperature, e.g., the amounts at 60℃ were about 1.5 times higher than those at 30℃. Initial transfer ratios (G_2-α-CD formed/α-G_2F consumed) of A-pullulanase and B-pullulanase were about 62% and 25% (at 40℃), and about 50% and 15% (at 20℃), respectively. The transfer rations of both A-pullulanase and B-pullulanase in the reaction using β-CD or γ-CD as acceptor also increased with a rise in temperature. The transfer ratios were little affected by any change in temperature or any kind of acceptor CDs, in the case of isoamylase, and were about 60%.
社団法人日本農芸化学会の論文
1988-07-23