Purification and Some Properties of Glycyrrhizinic Acid Hydrolase from Aspergillus niger GRM3
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概要
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We selected Aspergillus niger GRM3 because it secreted a novel enzyme that hydrolyzed glycyrrhizinic acid. The enzyme was purified to the electrophorctically homogeneous state and an activity more than 890-fold that of culture broth. The molecular weight of the enzyme was estimated to be 150, 000 by gel filtration and it was most active over the range of pH 4.1 to 4.5. The enzyme hydrolyzed glycyrrhizinic acid to produce glycyrrhetinic acid and D-glucuronyl-β-1, 2-Dglucuronic acid. The hydrolytic action of this enzyme was different from those of β-glucuronidases reported so far.
- 社団法人 日本農芸化学会の論文
著者
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KURAMOTO Takashi
Maruzen Kasei Co., Ltd.
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Okada Shigetaka
Osaka Municipal Technical Research Institute
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Muro Tetsuo
Osaka Municipal Technical Research Institute
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IMOTO Katsue
Maruzen Kasei Co., Ltd.
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