Structure of Di-O-α-Maltosyl Cyclodextrins Produced from α-Maltosylfluoride and Cyclodextrins(Biological Chemistry)

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概要

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• The structures of di-O-α-maltosyl β-cyclodextrins ((G_2)_2-β-CDs), which were produced from a-maltosylfluoride (α-G_2F) and cyclodextrin (CD) by the transfer action of debranching enzymes, were examined by the enzymic method using Bacillus subtilis saccharifying a-amylase (BSA). (G_2)_2-β-CD was converted to (G_1)_2-β-CD by treatment with glucoamylase before the examination. BSA completely hydrolyzed (G_1)_2-β-CD to produce glucose, 6^3-O-α-glucosylmaltotriose, and 6^3,6^5-di-O-α-glucosyl maltopentaose. (G_2)_2-β-CD was the mixture of 6^A,6^C-di-O-α-maltosyl β-CD and 6^A,6^D-di-O-α-maltosyl β-CD. The ratio of A,C/A,D in (G_2)_2-O-CD synthesized with Pseudomonas isoamylase and Aerobacter pullulanase were 40:60-45:55 and 30:70, respectively. The content of 6^A,6^C-di-O-α-maltosyl γ-CD in (G_2)_2-γ-CD synthesized by isoamylase was about 35%.

• 社団法人日本農芸化学会の論文
• 1990-10-23

著者

• KITAHATA Sumio

  Osaka Municipal Technical Research Institute

• Kitahata S

  Department Of Bioscience And Biotecnology Shinshu University

• Fujita Kahee

  Faculty Of Pharmaceutical Sciences Nagasaki University

• Okada S

  Osaka Municipal Technical Research Institute

• Okada Shigetaka

  Osaka Municipal Technical Research Institute

• Yoshimura Yoshimichi

  Tsukuba Research Laboratory Tokuyama Soda Co. Ltd.

• SATOMURA Yuka

  Faculty of Pharmaceutical Sciences, Fukuyama University

• Satomura Yuka

  Faculty Of Pharmaceutical Sciences Fukuyama University

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