Purification and Some Properties of a Trehalase from a Green Alga, Lobosphaera sp.
スポンサーリンク
概要
- 論文の詳細を見る
An unicellular green alga identified as Lobosphaera sp. by morphological observations was selected as a source of trehalase. The alga grew well heterotrophically and produced intracellular trehalase using Polypepton, yeast extract, and glycerol as nutrients. The enzyme was highly puritied by ammonium sulfate fractionation, column chromatography on DEAE-Toyopearl, Sepharose CL-4B, and SP-Toyopearl. The molecular mass was estimated to be 400kDa by gel filtration. SDS-PAGE indicated that the enzyme consisted of two subunits with a molecular mass range of 180-220kDa and it contained carbohydrates. The enzyme was most active at pH 5. 5 and at 65℃ and stable between pH 4-9 and below 65℃. Fe^<3+>inactivated the enzyme. Sucrose was a competitive inhibitor with a K_i of 7. 5mM. The enzyme specifically hydrolyzed trehalase with a Km of 0. 6mM.
- 社団法人日本農芸化学会の論文
- 1994-08-23
著者
-
MORIWAKI Masamitsu
San-Ei-Gen F.F.I., Inc.
-
KITAHATA Sumio
Osaka Municipal Technical Research Institute
-
NAKANO HIROFUMI
Osaka Municipal Technical Research Institute, and Yashiro Co, Ltd.,
-
Yoshizumi Hajime
Department Of Food Science And Nutrition Faculty Of Agriculture Kinki University
-
Yoshizumi Hajime
Institute For Fundamental Research Research Center
-
Yoshizumi Hajime
Kinki University
-
Kitahata S
Department Of Bioscience And Biotecnology Shinshu University
-
WASHINO Tutomu
Research and Development Section, San-Ei Gen F. F. I., Inc.
-
Washino Tutomu
Research And Development Section San-ei Gen F. F. I. Inc.
-
Washino T
San‐ei Gen F. F. I. Inc. Osaka Jpn
-
Washino Tsutomu
San-ei Gen F. F. I. Inc.
-
Kino Tetsuo
Kinki University
-
Nakano Hirofumi
Osaka Municipal Technical Research Institute
-
Moriwaki Masamitsu
Department Of Biotechnology The University Of Tokyo
-
Nakano Hirofumi
Department Of Chemistry Faculty Of Science Osaka University
-
Moriwaki Masamitsu
San-ei-gen F.f.i. Inc.
-
WASHINO Tsutomu
SAN-EI Chemical Industries Ltd.
関連論文
- Comparative digestion of cell wall components of alfalfa hay cubes between Sika deer (Cervus nippon) and cattle
- Seasonal changes in digestibility, passage rate and rumen fermentation of alfalfa hay in sika deer (Cervus nippon) under restricted feeding
- Comparison of digestibility, passage rate and rumen fermentation between sika deer (Cervus nippon) and cattle fed alfalfa hay cubes
- Effect of cyclodextrin diallyl maleate on methane production, ruminal fermentation and microbes in vitro and in vivo
- The characteristics of desaturation of Trichoderma sp. AM076 and formation of 9,12,15-hexadecatrienoic acid (16:3ω1) through Δ15 desaturation of 9,12-hexadecadienoic acid
- Microbial Synthesis of trans Isomer of Eicosapentaenoic Acid (EPA) from the Chemically Synthesized Trans Isomer of Linolenic Acid by a Δ12 Desaturase-defective Mutant of Mortierella alpina 1S-4(Microbiology & Fermentation Technology)
- Inhibitory Effects of Capsaicinoids on Fatty Acid Desaturation in a Rat Liver Cell Line(Food & Nutrition Science)
- C19 Odd-chain Polyunsaturated Fatty Acids (PUFAs) Are Metabolized to C21-PUFAs in a Rat Liver Cell Line, and Curcumin, Gallic Acid, and Their Related Compounds Inhibit Their Desaturation
- Formation of 8, 11, 14-Octadecatrienoic Acid (18:3 n-4) from Naturally Occurring Unique Fatty Acid, 9, 12-Hexadecadienoic Acid (16:2 n-4), in Animal Cell Cultures
- The Effects of Maternal Mild Protein Restriction on Stroke Incidence and Blood Pressure in Stroke-Prone Spontaneously Hypertensive Rats (SHRSP)
- Competitive Incorporation of Arachidonic Acid Analogs by Cultured Rat Keratinocytes
- Cell Age Distribution of Erythrocytes at the Incidence of Cerebral Stroke in Stroke-Prone Spontaneously Hypertensive Rats, and Their Glutathione Peroxidase Activity
- Increase of ruminal fiber digestion by cellobiose and a twin strain of Saccharomyces cerevisiae live cells in vitro
- Effect of α-cyclodextrin-allyl isothiocyanate on ruminal microbial methane production in vitro
- Effects of medium-chain fatty acid-cyclodextrin complexes on ruminal methane production in vitro
- 酵素処理イソクエルシトリン及び酵素処理ルチン(抽出物)の成分分析
- Preventative Effect of a Flavonoid, Enzymatically Modified Isoquercitrin on Ocular Symptoms of Japanese Cedar Pollinosis
- Effects of cyclodextrin-iodopropane complex on methane production, ruminal fermentation and microbes, digestibility and blood metabolites in steers
- Effect of propylene glycol and undegradable protein source on rumen fermentation, blood metabolism and milk production in lactating dairy cows
- Cloning and Expression in Escherichia coli of 2-Hydroxypropylphosphonic Acid Epoxidase from the Fosfomycin-producing Organism, Pseudomonas syringae PB-5123
- Further Study on the 1,4-α-Transglucosylation of Rubusoside, a Sweet Steviol-Bisglucoside from Rubus suavissimus(Organic Chemistry)
- Production of Stevioside and Rubusoside Derivatives by Transfructosylation of β-Fructofuranosidase(Biological Chemistry)
- Production of Rubusoside Derivatives by Transgalactosylation of Various α-Galactosidases(Biological Chemistry)
- Production of Rubusoside Derivatives by Transgalactosylation of Various β-Galactosidases(Biological Chemistry)
- Horseradish Peroxidase Degrades Lipid Hydroperoxides and Suppresses Lipid Peroxidation of Polyunsaturated Fatty Acids in the Presence of Phenolic Antioxidants(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Fatty Acid Specificity of Rhizopus delemar Lipase in Acidolysis
- High-level Secretion of a Rhizopus niveus Aspartic Proteinase in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
- Inhibition of β-Fructofuranosidases and α-Glucosidases by Synthetic Thio-fructofuranoside(Biochemistry & Molecular Biology)
- Enzymatic Synthesis of N-Acetylglucosaminyl-cyclodextrin by the Reverse Reaction of N-Acetylhexosaminidase from Jack Bean
- Synthesis of 6-O-α-D-Galactosyl α-Cyclodextrin by Coffee Bean α-Galactosidase
- Acceptor Specificity of Cyclodextrin Glycosyltransferase from Bacillus stearothermophilus and Synthesis of α-D-Glucosyl O-β-D-galactosyl-(1→4)-β-D-glucoside
- Galactosylation at Side Chains of Branched Cyclodextrins by Various β-Galactosidases
- Enzymic Synthesis of 4^5-O-β-Galactosyl-maltopentaose by Bacillus circulans β-Galactosidase(Biological Chemistry)
- Transfructosylation Catalyzed by β-Fructofurariosidase I from Arthrobacter sp. K-1(Biological Chemistry)
- Purification and Some Properties of β-Fructofuranosidase I from Arthrobacter sp. K-1(Biological Chemistry)
- Pyrronamycin A and B, Novel Antitumor Antibiotics Containing Pyrrole-amide Repeating Unit, Produced by Streptomyces sp.
- UCT1072s, New Antitumor Antibiotics with Topoisomerase II Mediated DNA Cleavage Activity, from Aspergillus sp.
- Enzymatically Modified Isoquercitrin, α-Oligoglucosyl Quercetin 3-O-Glucoside, Is Absorbed More Easily than Other Quercetin Glycosides or Aglycone after Oral Administration in Rats
- Synthesis of Novel Heterobranched β-Cyclodextrins Having β-D-N-Acetylglucosaminyl-Maltotriose on the Side Chain
- Preparation and Characterization of Novel Branched β-Cyclodextrins Having β-D-Galactose Residues on the Non-reducing Terminal of the Side Chains and Their Specific Interactions with Peanut (Arachis hypogaed) Agglutinin
- Synthesis of Glycosyl Glycerol by Cyclodextrin Glucanotransferases(ENZYMOLOGY,PROTEIN ENGINEERING,AND ENZYME TECHNOLOGY)
- Hydrolysis of β-Glucosyl Ester Linkage of p-Hydroxybenzoyl β-D-Glucose, a Chemically Synthesized Glucoside, by β-Glucosidases
- Synthesis of Novel Heterobranched β-Cyclodextrins from α-D-Mannosyl Maltotriose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products
- Hydrolysis of β-Galactosyl Ester Linkage by β-Galactosidases
- Transfructosylation of Thiol Group by β-Fructofuranosidases
- Synthesis of Novel Heterobranched β-Cyclodextrins from 4^2-O-β-D-Galactosyl-Maltose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products
- Galactosylation of Thiol Group by β-Galactosidase
- Purification and Some Properties of a β-Glucosidase from Flavobacterium johnsonae
- Purification and Characterization of a Novel β-Glucosidase from Clavibacter michiganense That Hydrolyzes Glucosyl Ester Linkage in Steviol Glycosides
- Enzymatic Synthesis of Oligosaccharides Containing Galβ→4Gal Disaccharide at the Non-Reducing End Using β-Galactanase from Penicillium citrinum
- Enzymatic Synthesis of Mannosyl-cyclodextrin by α-Mannosidase from Jack Bean
- Synthesis of 2-Deoxy-glucooligosaccharides through Condensation of 2-Deoxy-D-glucose by Glucoamylase and α-Glucosidase
- Esterification of Glycosides by a Mono- and Diacylglycerol Lipase from Penicillium camembertii and Comparison of the Products with Candida cylindracea Lipase
- Reaction Conditions for the Production of γ-Cyclodextrin by Cyclodextrin Glucano-transferase from Brevibacterium sp. No.9605
- Transgalactosylation Catalyzed by α-Galactosidase from Candida guilliermondii H-404
- Enzymatic Synthesis of α-Linked Galactooligosaccharides Using the Reverse Reaction of a Cell-bound α-Galactosidase from Candida guilliermondii H-404
- Evaluation of Chiral Amino Acid Discrimination by a Permethylated Cyclic Tetrasaccharide, cyclo-{→6)-α-D-Glcp-(1→3)-α-D-Glcp-(1→6)-α-D-Glcp-(1→3)-α-D-Glcp-(1→}, Using FAB Mass Spectrometry
- Glucoamylase Produced by Rhizopus and by a Recombinant Yeast Containing the Rhizopus Glucoamylase Gene(Biological Chemistry)
- Galactosylation of Cyclodextrins and Branched Cyclodextrins by α-Galactosidases
- Acceptor Specificities of α-Mannosidases from Jack Bean and Almond, and Trans-mannosylation of Branched Cyclodextrins
- Purification and Some Properties of α-Galactosidase from Candida guilliermondii H-404
- Purification and Some Properties of α-Galactosidase from Pseudomonas fluorescens H-601(Biological Chemistry)
- Purification and Some Properties of Dextrin Dextranase from Acetobacter capsulatus ATCC 11894
- A Comparison of GUS Activity after Liquid- and Air-Heat Shock Treatments in Transgenic Nicotiana plumbaginifolia Harboring the Arabidopsis HSP18.2 Promoter-GUS Chimeric Gene
- Suppressed Phenylalanine Ammonia-Lyase Activity after Heat Shock in Transgenic Nicotiana plumbaginifolia Containing an Arabidopsis HSP18.2-Parsley PAL2 Chimera Gene
- Characterization of Dimers of Hydroquinone Glucosides Produced by Peroxidase-Catalyzed Polymerization
- Purification and Characterization of a Carbohydrate:Acceptor Oxidoreductase from Paraconiothyrium sp. That Produces Lactobionic Acid Efficiently
- Purification and Characterization of a Novel α-Glucuronidase from Aspergillus niger Specific for O-α-D-Glucosyluronic Acid α-D-Glucosiduronic Acid
- Production of Structured Lipid Containing Docosahexaenoic and Caprylic Acids Using Immobilized Rhizopus delemar Lipase
- Purification and Some Properties of a Levanase from Streptomyces sp. No. 7-3(Biological Chemistry)
- Effects of protozoa on the antioxidant activity in the ruminal fluid and blood plasma of cattle
- Mouse N-Acetylgalatosamine 4-Sulfotransferases-1 and -2. Molecular Cloning, Expression, Chromosomal Mapping and Detection of Their Activity with GalNAcβ1-4GlcNAcβ1-octyl
- Chemical Structures of Hetero-oligosaccharides Produced by Arthrobacter sp. K-1β- Fructofuranosidase
- The Acceptor Specificity of Amylomaltase from Escherichia coli IFO 3806(Biological Chemistry)
- Transfer Reaction Catalyzed by Exo-β-1,4-galactanase from Bacillus subtilis(Biological Chemistry)
- Formation of Transfer Products from Soybean Arabinogalactan and Glycerol by Galactanase from Penicillium citrinum(Biological Chemistry)
- Transfer Reaction Catalyzed by Galactanase from Penicillium citrinum(Biological Chemistry)
- Activation of Galactanase from Penicillium citrinum by HgCl_2 and KCl(Biological Chemistry)
- Action of Galactanase from Penicillium citrinum on o-Nitrophenyl-β-D-galactopyranoside(Biological Chemistry)
- Lipase-Catalyzed Esterification of Mono- and Oligoglycosides with Various Fatty Acids
- Purification and Characterization of a New Type of α-Glucosidase from Paecilomyces lilacinus That Has Transglucosylation Activity to Produce α-1,3- and α-1,2-Linked Oligosaccharides(Biochemistry & Molecular Biology)
- Syntheses of Glucosides with Trimethylolpropane and Two Related Polyol Moieties by Cyclodextrin Glucanotransferase and Their Esterification by Lipase
- Characterization of Dimers of Hydroquinone Glucosides Produced by Peroxidase-Catalyzed Polymerization
- Enzymatic Synthesis of (+)Catechin-α-glucoside and Its Effect on Tyrosinase Activity
- Prediction of Stroke Lesions in Stroke-prone Spontaneously Hypertensive Rats by Glutathione Peroxidase in Erythrocytes
- Structure of Di-O-α-Maltosyl Cyclodextrins Produced from α-Maltosylfluoride and Cyclodextrins(Biological Chemistry)
- Effects of Temperature on 6-O-α-Maltosyl Cyclodextrin Production from α-Maltosylfluoride and Cyclodextrins(Biological Chemistry)
- Purification and Some Properties of a New Levanase from Bacillus sp. No. 71
- Purification and Some Properties of a Trehalase from a Green Alga, Lobosphaera sp.
- Purification and Characterization of a Carbohydrate : Acceptor Oxidoreductase from Paraconiothyrium sp. That Produces Lactobionic Acid Efficiently
- UCH9, a New Antitumor Antibiotic Produced by Streptomyces II. Structure Elucidation of UCH9 by Mass and NMR Spectroscopy
- Purification and Properties of Cyclodextrin Glucanotransferase from Brevibacterium sp. No. 9605
- Formation of Trehalose and Its 2-Deoxy Analogs through Condensation by a Trehalase from Lobosphaera sp.
- Esterification of Glycosides with Glycerol ant Trimethylolpropane Moieties by Candida cylidracea Lipase(Biological Chemistry)
- Purification and Some Properties of Amylomaltase from Escherichia coli IFO 3806(Biological Chemistry)
- Optimization of Lactobionic Acid Production by Acetobacter orientalis Isolated from Caucasian Fermented Milk, "Caspian Sea Yogurt"
- Inhibitory Effects of Whisky Polyphenols on Melanogenesis in Mouse B16 Melanoma Cells
- Effect of supplementation of soy sauce oil and Ca salts of fatty acids on rumen fermentation, milk production and conjugated linoleic acid in milk of dairy cows
- Purification and SomeProperties of Cyclodextrinase from Bacillus coagulans
- Enzymic Transglucosylation of Rubusoside and the Structure-Sweetness Relationship of Steviol-Bisglycosides