Suppressed Phenylalanine Ammonia-Lyase Activity after Heat Shock in Transgenic Nicotiana plumbaginifolia Containing an Arabidopsis HSP18.2-Parsley PAL2 Chimera Gene

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概要

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• The activity of phenylalanine ammonia-lyase(PAL ; EC 4.3.1.5)after heat shock(HS)in leaves and buds of transgenic Nicotiana plumbaginifolia containing an Arabidopsis HSP18.2 promoter-parsley phenylalanine ammonia-lyase 2(HSP18.2-PAL2)chimera gene was examined. Immediately after HS treatment at 44℃ for 5h, the PAL activity in both transgenic and normal(untransformed)plants was 35-38% lower than that before HS. At normal temperature(25-26℃), the PAL activity recovered within 5h of ending the HS treatment in normal plants, but not until 12-24h in transgenic plants containing the HSP18.2-PAL2 gene. Reverse transcription-polymerase chain reaction(RT-PCR)analysis revealed the presence of parsley PAL2 mRNA in transgenic plants, which remained for 8-12h following 5-h HS at 44℃ ; the mRNA was not observed before HS. The content of chlorogenic acid(CGA ; 3-caffeoylquinic acid)decreased drastically 8-12h after HS in transgenic plants, but only slightly in normal plants. Thus, the decrease in PAL activity accompanied by expression of the parsley PAL2 gene after HS treatment corresponded to the decrease in CGA synthesis. These results might be attributed to post-transcriptional degradation of endogenous PAL mRNA triggered by transcription of the transgene.

• 社団法人日本生物工学会の論文
• 1999-05-25

著者

• YAMAKAWA Takashi

  Department of Global Agricultural Sciences, The University of Tokyo

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• KODAMA TOHRU

  Faculty of Textile Science and Technology, Shinshu University

• Igarashi Y

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• Yamakawa Takashi

  Laboratory Of Plant Biotechnology Department Of Global Agricultural Sciences The University Of Tokyo

• Yamakawa Takashi

  Department Of Biotechnology The University Of Tokyo:(present Address)department Of Global Agricultur

• Yamakawa Takashi

  Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of

• Yamakawa Takashi

  Biotechnology Research Center Toyama Prefectural University

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamakawa T

  Laboratory Of Plant Biotechnology Department Of Global Agricultural Sciences The University Of Tokyo

• MORIWAKI Masamitsu

  Department of Biotechnology, The University of Tokyo

• WASHINO Tutomu

  Research and Development Section, San-Ei Gen F. F. I., Inc.

• WASHINO TUTOMU

  Development Section, San-Ei Gen F.F.I., Inc.

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• Washino Tutomu

  Research And Development Section San-ei Gen F. F. I. Inc.

• Washino T

  San‐ei Gen F. F. I. Inc. Osaka Jpn

• Moriwaki Masamitsu

  Department Of Biotechnology The University Of Tokyo

• Yamakawa Takashi

  Department Of Agricultural Chemistry University Of Tokyo

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