Structure and ANR-Dependent Transcription of the nir Genes for Denitritication from Pseudomonas aeruginosa
スポンサーリンク
概要
- 論文の詳細を見る
In the denitrification gene cluster from Pseudomonas aeruginosa, an operon encoding three open reading frames (nirQ, ORF2, ORF3) was upstream of the structural gene for nitrite reductase (nirS) as a divergent transcriptional organization. A nucleotide-binding protein encoded by nirQ was 76o/o identical to the Pseudomonas stutzeri nirQ gene product, which was shown to be necessary for activating nitrite and nitric oxide reductases. The gene product of ORF2 was homologous to subunit III of cytochrome oxidases. The nirQ gene was transcribed under denitrifying conditions. The intergenic region of nirS and nirQ has only one binding motif for ANR, a regulatory protein for anaerobic gene expression correspond to FNR in E. coli. Complementation analyses showed that the transcription of both nirS and nirQ completely depended on ANR.
- 社団法人日本農芸化学会の論文
- 1994-07-23
著者
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ARAI Hiroyuki
Department of Biotechnology, The University of Tokyo
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IGARASHI Yasuo
Department of Biotechnology, The University of Tokyo
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Igarashi Y
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Igarashi Yasuo
University Of Tokyo
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Igarashi Yasuo
Department Of Biotechnology University Of Tokyo
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KODAMA Tohru
Department of Textile Science and Technology, Shinshu University
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Arai Hiroyuki
Graduate School Of Biotechnology The Univ. Of Tokyo
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Igarashi Y
Univ. Tokyo Tokyo Jpn
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Arai H
Univ. Tokyo Tokyo Jpn
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Kodama Tohru
Department Of Agricultural Chemistry The University Of Tokyo
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Kokubo Tetsuro
Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An
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Arai H
Graduate School Of Biotechnology The Univ. Of Tokyo
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Kodama Tohru
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Arai Hiroyuki
Department Of Biotechnology The University Of Tokyo
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Arai Hiroyuki
Department Of Applied Chemistry Tokai University
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