Growth Characteristics and Dense Culture of a Thermophilic Cyanobacterium, Chroococcidiopsis sp. Strain TS-821

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概要

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• A thermophilic cyanobacterium, Chroococcidiopsis sp. strain TS-821,was cultivated under illumination at various intensities of and at various concentrations of medium components in a 1-l photobioreactor. The specific growth rate and the dry cell weight at the end of cultivation were determined. On the basis of the results obtained, cultivation under the optimum conditions was carried out for achieving a dense culture. Cultivation was also done under conditions of CO_2 supply in two different modes : (i) 10% CO_2 throughout, and (ii) 10% at the beginning and then 20% at the termination of linear growth. The dry cell yield per liter at the end of the culture was 70% higher than that in the case of standard culture. The total sugar content of the cells reached 30% of the dry cell weight at the end of the dense culture, which is higher than that of other cyanobacterium, Synechococcus elongatus PCC 6301. The supernatant of a buffer suspension of cells which had been collected at the end of the dense culture and freeze-dried was clear blue. The absorption spectrum of this supernatant was measured and compared with those of the cell extract and of a phycobiliprotein extracted from cell of this strain. The wavelengths of two absorption maxima from the supernatant coincided with those from the phycobiliprotein. The results show that the phycobiliprotein can easily be excreted from the cells. the results of polyacrylamide gel electrophoresis and spectrophotometrical analyses suggest that the excreted phycobiliprotein is a phycocyanin. Furthermore, when total protein, lipid, and sugar contents were measured without removal of the culture medium, the total sugar content was about 50% of the cell component. This indicates that the sugar was excreted into the medium.

• 社団法人日本生物工学会の論文
• 1997-06-25

著者

• ISHII Masaharu

  Department of Biotechnology, The University of Tokyo

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• 石井 正浩

  University Of Tokyo

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• PEERAPORNPISAL Yuwadee

  Department of Biology, Chiangmai University

• ISHIDA TATSUYA

  Department of Biotechnology, University of Tokyo

• HASEGAWA NORIO

  Department of Biotechnology, University of Tokyo

• HAYASHI NOBUHIRO

  Department of Biotechnology, University of Tokyo

• KODAMA TOHRU

  (Present address)Faculty of Textile Science and Technology, Shinshu University

• Ishii Masaharu

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo

• Igarashi Y

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B

• Hasegawa Norio

  Department Of Urology Jikei University School Of Medicine

• Ishii Masaharu

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Ishida Tatsuya

  Department Of Biotechnology University Of Tokyo

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• Hayashi Norihiro

  Department Of Urology Jikei University School Of Medicine

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• Hasegawa N

  Hitachi Ltd. Tokyo Jpn

• Hasegawa N

  Univ. Tokyo Tokyo Jpn

• Hasegawa Norio

  Department Of Biotechnology University Of Tokyo

• Hasegawa Norio

  Department Of Material Physics Faculty Of Engineering Science Osaka University

• Iwasaki T

  Department Of Biochemistry And Molecular Biology Nippon Medical School

• Hayashi N

  Department Of Biotechnology University Of Tokyo

• Peerapornpisal Y

  Department Of Biology Chiangmai University

• Peerapornpisal Yuwadee

  Department Of Biology Faculty Of Science Chiang Mai University

• Hayashi Nobuhiro

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• ISHII Masaharu

  Department of Agricultural Chemistry, The University of Tokyo

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