A Non-radioisotopic Anion-exchange Chromatographic Method to Measure the CO_2/O_2 Specificity Factor for Ribulose Bisphosphate Carboxylase/Oxygenase

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概要

• 論文の詳細を見る

• A non-radioisotopic anion-exchange ion chromatographic method for measuring the carboxylation/oxygenation specificity (τ) of ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) is presented. The assay measures the amounts of fixation products at varying [CO_2]/[O_2] ratios to measure the relative rates of CO_2 and O_2 fixation reactions. The amount of 3-phosphoglycerate (3-PGA) and phosphoglycolate (PG) in the reaction mixture were measured with a conductivity detector and the specific factor was calculated using the following equations: v_c=([3-PGA]-[PG])/2 and v_o= [PG]. By this method, specificity factors for RubisCOs were measured without using radioactive reagents.

• 社団法人日本農芸化学会の論文
• 1996-06-23

著者

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• KODAMA Tohru

  Department of Textile Science and Technology, Shinshu University

• Igarashi Y

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B

• OUCHIYAMA Naoki

  Kurume Laboratory, Chemicals Evaluation and Research Institute (CERI)

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kodama Tohru

  Department Of Agricultural Chemistry The University Of Tokyo

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• OGUNI ASAKO

  Department of Biotechnology, University of Tokyo

• YAGUCHI TOSHIAKI

  Institute for Fundamental Research, Suntory Limited

• Oguni Asako

  Department Of Biotechnology University Of Tokyo

• Yaguchi Toshiaki

  Institute For Fundamental Research Suntory Limited

• Kodama Tohru

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Ouchiyama Naoki

  Kurume Laboratory Chemicals Evaluation And Research Institute (ceri)

• Ouchiyama Naoki

  Kurume Research Laboratories Chemicals Inspection & Testing Institute

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