Changes in the gene expression profile of Acetobacter aceti during growth on ethanol(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Acetobacter aceti NBRC 14818 shows a diauxic growth profile and temporarily accumulates acetate when grown in medium containing ethanol. However, the mechanisms underlying the metabolic switching between the incomplete oxidation of ethanol and overoxidation of acetate, and the control of stress resistance systems in A. aceti cells grown on ethanol are not fully understood. In this study, time-dependent transcriptome changes in cells during growth on ethanol were analyzed by DNA microarray. In A. aceti, ethanol is oxidized to acetate via acetaldehyde by sequential reactions of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). We found that the genes encoding pyrroloquinoline quinone-dependent ADH, membrane-bound ALDH, and two NAD^+_ADHs were expressed constitutively in cells throughout the culture period. In contrast, the expression levels of genes encoding tricarboxylic acid (TCA) cycle enzymes were low during acetate accumulation until ethanol was consumed, but were significantly upregulated after the accumulated acetate was started to be consumed. This result suggests that changes in the carbon metabolic flow through the TCA cycle are important for the metabolic switching from acetate accumulation to the overoxidation of acetate. In addition, the genes for glyoxylate pathway enzymes were significantly upregulated soon after the cells began oxidizing ethanol, indicating that this pathway is important for the utilization of ethanol as a carbon source.
著者
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石井 正治
東京大学大学院農学生命科学研究科
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石井 正治
東京大学大学院農学生命科学研究科応用工学専攻
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Igarashi Y
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Igarashi Yasuo
University Of Tokyo
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Ishii M
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Ishii Masaharu
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo
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Igarashi Y
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B
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Ishii Masaharu
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Arai Hiroyuki
Graduate School Of Biotechnology The Univ. Of Tokyo
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Igarashi Y
Univ. Tokyo Tokyo Jpn
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Arai H
Univ. Tokyo Tokyo Jpn
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Arai H
Graduate School Of Biotechnology The Univ. Of Tokyo
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Arai Hiroyuki
Department Of Biotechnology The University Of Tokyo
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Sakurai Kenta
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of To
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Sakurai Kenta
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Arai Hiroyuki
Department Of Applied Chemistry Tokai University
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ISHII Masaharu
Department of Agricultural Chemistry, The University of Tokyo
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