Denaturing Gradient Gel Electrophoresis Analyses of Microbial Community from Field-Scale Composter

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概要

• 論文の詳細を見る

• The diversity of microbial community during the decomposition of waste in a field-scale composter (Hazaka system) was investigated by denaturing gradient gel electrophoresis (DGGE). The composter operates at a high temperature through a self-heating system, creating a thermophilic (60-76℃) stage during the initial phase and a mesophilic (45℃) stage towards the later phase of the composting period. The pH of the system (pH 7.75-8.10) did not vary significantly during the process while moisture content was reduced from 48.8% to 25.1%. DGGE and 16S rDNA analyses showed that the following genera were found throughout the process:Propionibacterium sp., Methylobacterium sp., Pseudomonas sp., and Bradyrhizobium sp. Different Bacillus spp.thrive at the thermophilic or the mesophilic stage while Clostridium sp.was only found at the initial phase of the process. Staphylococcus sp.and Caulobacter sp.or Brevundimonas sp.existed during the later phase of the composting period.

• 社団法人日本生物工学会の論文
• 2001-02-25

著者

• ISHII Masaharu

  Department of Biotechnology, The University of Tokyo

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• 石井 正浩

  University Of Tokyo

• 石井 正治

  東京大学大学院農学生命科学研究科応用工学専攻

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• PEDRO MANNIX

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, the University of To

• HARUTA SHIN

  Department of Biotechnology, Graduate School of Agricultural and Life Sciences, the University of To

• Ishii M

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo

• Ishii Masaharu

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo

• Igarashi Y

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B

• HAZAKA MASARU

  Kennan Eisei Kogyo Co.,Hazaka Plant Laboratory

• Haruta Shin

  Division Of Microbial Chemistry Faculty Of Pharmaceutical Science Chiba University

• Ishii Masaharu

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Hazaka Masaru

  Kennan Eisei Kogyo Co.

• Pedro Mannix

  Department Of Biotechnology Graduate School Of Agricultural And Life Science The University Of Tokyo

• SHIMADA RUMIKO

  Kennan Eisei Kogyo Co., Hazaka Plant Laboratory

• YOSHIDA CHIE

  Kennan Eisei Kogyo Co., Hazaka Plant Laboratory

• HIURA KOICHIRO

  Kennan Eisei Kogyo Co., Hazaka Plant Laboratory

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Yoshida Chie

  Kennan Eisei Kogyo Co. Hazaka Plant Laboratory

• Hiura Koichiro

  Kennan Eisei Kogyo Co. Hazaka Plant Laboratory

• Shimada Rumiko

  Kennan Eisei Kogyo Co. Hazaka Plant Laboratory

• Ishii Masaharu

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Haruta Shin

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Hasegawa S

  Department Of Biomolecular Engineering Graduate School Of Engineering Thhoku University

• Haruta Shin

  Department Of Biological Sciences Tokyo Metropolitan University

• ISHII Masaharu

  Department of Agricultural Chemistry, The University of Tokyo

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