Effects of Aeration on Tropane Alkaloid Production by Transformed Root of Atropa belladonna in Flask Cultures
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概要
- 論文の詳細を見る
Transformed roots of Atropa belladonna strain M8 were cultured in shaking flasks aerated with normal air at 1 vvm, capped with aluminum foil, or capped with a silicon rubber stopper, to investigate the effects of aeration on root growth and on tropane alkaloid production and composition. After four weeks, the tropane alkaloid productivity of roots grown in flasks aerated at 1 vvm reached 52.9 mg/l, and the individual tropane alkaloid contents in the roots were, in mg/l dry wt., (-)-hyoscyamine 4.8, 6β-hydroxyhyoscyamine 1.4, littorine 1.2, and scopolamine 1.0. These results indicate that aeration at 1 vvm air has an important effect on the control of the tropane alkaloid composition in root cultures. In particular, the scopolamine ratio increased from 5.7 to 11.9% of the total alkaloids accumulated in the belladonna cultured roots.
- 社団法人日本生物工学会の論文
- 1998-12-25
著者
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YAMAKAWA Takashi
Department of Global Agricultural Sciences, The University of Tokyo
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IGARASHI Yasuo
Department of Biotechnology, The University of Tokyo
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Igarashi Y
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Igarashi Yasuo
University Of Tokyo
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KODAMA TOHRU
Faculty of Textile Science and Technology, Shinshu University
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SHIMOMURA Koichiro
Tsukuba Medicinal Plant Research Station, National Institute of Health Sciences
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Igarashi Y
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B
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Igarashi Y
Univ. Tokyo Tokyo Jpn
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SHIMOMURA Koichiro
Faculty of Life Sciences, Toyo University
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Yamakawa Takashi
Laboratory Of Plant Biotechnology Department Of Global Agricultural Sciences The University Of Tokyo
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Yamakawa Takashi
Department Of Biotechnology The University Of Tokyo:(present Address)department Of Global Agricultur
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Yamakawa Takashi
Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of
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Yamakawa Takashi
Biotechnology Research Center Toyama Prefectural University
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Shimomura Koichiro
Tsukuba Medicinal Plant Research Station National Institute Of Health Sciences
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Yamakawa T
Laboratory Of Plant Biotechnology Department Of Global Agricultural Sciences The University Of Tokyo
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LEE KUNG-TA
Department of Biotechnology, The University of Tokyo
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Kodama T
Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And
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Kokubo Tetsuro
Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An
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Lee Kung-ta
Department Of Biotechnology The University Of Tokyo:(present Address)department Of Agricultural Chem
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Shimomura K
National Inst. Of Health Sci. Ibaraki Jpn
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Yamakawa Takashi
Department Of Agricultural Chemistry University Of Tokyo
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Shimomura Koichiro
Tsukuba Medicinal Plant Research Station National Institute Of Health Science
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