Two Tandemly Arranged Ferredoxin Genes in the Hydrogenobacter thermophilus Genome : Comparative Characterization of the Recombinant [4Fe-4S] Ferredoxins

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概要

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• A thermophilic, obligately chemolithoautotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6, assimilates carbon dioxide via the reductive tricarboxylic acid cycle. A gene encoding a ferredoxin involved in this cycle as an electron donor (HtFd1) was cloned and sequenced. Interestingly, another ferredoxin gene (encoding HtFd2) was found in tandem with the HtFd1 gene. These two ferredoxin genes overlapped by four bp, and transcriptional analysis revealed that they are co-transcribed as an operon. The deduced amino acid sequences of HtFd1 and HtFd2 were 42.9% identical and each contained four cysteine residues that serve as probable ligands to an iron-sulfur cluster. Spectroscopic analyses of the purified recombinant ferredoxins heterologously expressed in Escherichia coli indicated that each ferredoxin contains a single [4Fe–4S]2+⁄1+ cluster.

• 社団法人 日本農芸化学会の論文
• 2005-06-23

著者

• ARAI Hiroyuki

  Department of Biotechnology, The University of Tokyo

• ISHII Masaharu

  Department of Biotechnology, The University of Tokyo

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• Yamamoto Masahiro

  Department Of Surgery Teikyo University School Of Medicine

• 石井 正浩

  University Of Tokyo

• 石井 正治

  東京大学大学院農学生命科学研究科応用工学専攻

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• IKEDA Takeshi

  Department of Biotechnology, University of Tokyo

• OHMORI Daijiro

  Department of Chemistry, School of Medicine, Juntendo University

• Ishii Masaharu

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo

• Igarashi Y

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B

• Ishii Masaharu

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Igarashi Yasuo

  Department Of Biotechnology The University Of Tokyo

• Arai Hiroyuki

  Graduate School Of Biotechnology The Univ. Of Tokyo

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• Igarashi Yasuo

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Arai H

  Univ. Tokyo Tokyo Jpn

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Arai H

  Graduate School Of Biotechnology The Univ. Of Tokyo

• Yamamoto Masahiro

  Department Of Earth Sciences Okayama University

• Ohmori Daijiro

  Department Of Chemistry Juntendo University School Of Medicine

• Ohmori Daijiro

  Department Of Chemistry School Of Medicine Juntendo University

• Ikeda Takeshi

  Department Of Biotechnology University Of Tokyo

• Arai Hiroyuki

  Department Of Biotechnology The University Of Tokyo

• Yamamoto Masahiro

  Department Of Biotechnology The University Of Tokyo

• Yamamoto Masahiro

  Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University

• Arai Hiroyuki

  Department Of Applied Chemistry Tokai University

• ISHII Masaharu

  Department of Agricultural Chemistry, The University of Tokyo

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