Methanogenic pathway and community structure in a thermophilic anaerobic digestion process of organic solid waste(ENVIRONMENTAL BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
The methanogenic pathway and microbial community in a thermophilic anaerobic digestion process of organic solid waste were investigated in a continuous-flow stirred-tank reactor using artificial garbage slurry as a feedstock. The decomposition pathway of acetate, a significant precursor of CH_4 and a key intermediate metabolite in the anaerobic digestion process, was analyzed by using stable isotopes. A tracer experiment using ^<13>C-labeled acetate revealed that approximately 80% of the acetate was decomposed via a non-aceticlastic oxidative pathway, whereas the remainder was converted to methane via an aceticlastic pathway. Archaeal 16S rRNA analyses demonstrated that the hydrogenotrophic methanogens Methanoculleus spp. accounted for >90% of detected methanogens, and the aceticlastic methanogens Methanosarcina spp. were the minor constituents. The clone library targeting bacterial 16S rRNA indicated the predominance of the novel Thermotogales bacterium (relative abundance: 〜53%), which is related to anaerobic acetate oxidizer Thermotoga lettingae TMO, although the sequence similarity was low. Uncultured bacteria that phylogenetically belong to municipal solid waste cluster I were also predominant in the microflora (〜30%). These results imply that the microbial community in the thermophilic degrading process of organic solid waste consists exclusively of unidentified bacteria, which efficiently remove acetate through a non-aceticlastic oxidative pathway.
著者
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ISHII Masaharu
Department of Biotechnology, The University of Tokyo
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IGARASHI Yasuo
Department of Biotechnology, The University of Tokyo
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石井 正治
東京大学大学院農学生命科学研究科
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石井 正浩
University Of Tokyo
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石井 正治
東京大学大学院農学生命科学研究科応用工学専攻
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Igarashi Y
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Igarashi Yasuo
University Of Tokyo
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Hori Tomoyuki
Department of Internal Medicine, Kitasato University School of Medicine
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HARUTA SHIN
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, the University of To
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Ishii M
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Ishii Masaharu
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo
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Igarashi Y
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B
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Ueno Yoshiyuki
Kajima Technical Research Institute
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SASAKI Daisuke
Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences
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Haruta Shin
Division Of Microbial Chemistry Faculty Of Pharmaceutical Science Chiba University
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Ishii Masaharu
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Igarashi Yasuo
Department Of Biotechnology The University Of Tokyo
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Ueno Yoshiyuki
Marine Biotechnology Institute Kamaishi Laboratories
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Igarashi Y
Univ. Tokyo Tokyo Jpn
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Igarashi Yasuo
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Ueno Y
Kajima Technical Research Institute
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Ishii Masaharu
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Hori Tomoyuki
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Haruta Shin
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Sasaki Daisuke
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Sasaki Daisuke
Environmental Science Research Laboratory Central Research Institute Of Electric Power Industry (cri
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Hasegawa S
Department Of Biomolecular Engineering Graduate School Of Engineering Thhoku University
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Haruta Shin
Department Of Biological Sciences Tokyo Metropolitan University
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ISHII Masaharu
Department of Agricultural Chemistry, The University of Tokyo
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SASAKI Daisuke
Department of Aerospace Engineering, Tohoku Univeristy
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