Purification, Characterization, and Gene Cloning of Thermophilic Cytochrome cd_1 Nitrite Reductase from Hydrogenobacter thermophilus TK-6(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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A thermophilic, chemolithoautotrophic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus TK-6 can grow autotrophically under anaerobic conditions by denitrification. One of the denitrification enzymes, cytochrome cd_1 nitrite reductase, was isolated and its gene was cloned from strain TK-6. The subunit molecular mass of the purified enzyme was 61.5 kDa and the isoelectric point was determined to be 9.3. The optimum temperature and pH for the enzymatic reaction were 70-75℃ and 6.5-7.0, respectively. The structural gene for the enzyme, nirS, is probably transcribed as a hexacistronic operon with the following genes encoding a putative diheme cytochrome c and the proteins required for biosynthesis of heme d_1. The NirS sequence was phylogenetically distinct from those of proteobacteria. The consensus -35 and -10 sequences were found in the putative nirS promoter region, but the consensus sequences for the DNR/NnrR-type or the NorR/FhpR-type nitric oxide sensing regulators were not found in this region.
- 2006-05-25
著者
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SUZUKI Miho
Department of Biotechnology, The University of Tokyo
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ARAI Hiroyuki
Department of Biotechnology, The University of Tokyo
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ISHII Masaharu
Department of Biotechnology, The University of Tokyo
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IGARASHI Yasuo
Department of Biotechnology, The University of Tokyo
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Hirai Tadao
Department of Biotechnology, The University of Tokyo
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石井 正浩
University Of Tokyo
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石井 正治
東京大学大学院農学生命科学研究科応用工学専攻
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Igarashi Y
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Igarashi Yasuo
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo
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Igarashi Yasuo
University Of Tokyo
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Hirai Tadao
Department Of Biotechnology The University Of Tokyo
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Ishii Masaharu
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo
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Igarashi Y
Dep. Of Biotechnology Graduate School Of Agricultural And Life Sci. The Univ. Of Tokyo Yayoi 1-1-1 B
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Suzuki Miho
Department Of Biotechnology The University Of Tokyo
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Ishii Masaharu
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Igarashi Yasuo
Department Of Biotechnology The University Of Tokyo
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Arai Hiroyuki
Graduate School Of Biotechnology The Univ. Of Tokyo
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Igarashi Y
Univ. Tokyo Tokyo Jpn
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Igarashi Yasuo
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Arai H
Univ. Tokyo Tokyo Jpn
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Igarashi Yasuo
Department Of Agricultural Chemistry The University Of Tokyo
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Ishii Masaharu
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Arai H
Graduate School Of Biotechnology The Univ. Of Tokyo
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Suzuki Miho
Department Of Biology Faculty Of Science Nagoya University
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Arai Hiroyuki
Department Of Biotechnology The University Of Tokyo
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Arai Hiroyuki
Department Of Applied Chemistry Tokai University
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ISHII Masaharu
Department of Agricultural Chemistry, The University of Tokyo
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