Purification and Characterization of Membrane-bound Hydrogenase from a Thermophilic Hydrogen-Oxidizing Bacterium, Pseudomonas hydrogenothermophila Strain TH-1

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概要

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• A membrane-bound hydrogenase was purified aerobically by one step using a hydroxyapatite column after solubilization by acetone treatment from a thermophilic hydrogen-oxidizing bacterium, Pseudomonas hydrogenothermophila strain TH-1. The enzyme consists of two polypeptides of 63 and 31 kDa, respectively. The amino-terminal amino acid sequences of both subunits were homologous to membrane-bound type [Ni-Fe] hydrogenases from other origins. The thermostability under a hydrogen gas atmosphere is highly stable at 50℃, which is the optimum temperature for the cell growth.

• 社団法人日本農芸化学会の論文
• 1995-05-23

著者

• ISHII Masaharu

  Department of Biotechnology, The University of Tokyo

• IGARASHI Yasuo

  Department of Biotechnology, The University of Tokyo

• Igarashi Y

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Igarashi Yasuo

  University Of Tokyo

• KODAMA Tohru

  Department of Textile Science and Technology, Shinshu University

• Ono Tetsuya

  Department of Internal Medicine, Kure Kyosai Hospital

• Yoon Ki

  Department Of Ceramic Engineering Yonsei University

• Ishii M

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences University Of Tokyo

• Ishii Masaharu

  Dep. Of Biotechnology Graduate School Of Agricultural And Life Sciences Univ. Of Tokyo

• Ishii Masaharu

  Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky

• Yoon Ki

  Department Of Biotechnology The University Of Tokyo

• Igarashi Y

  Univ. Tokyo Tokyo Jpn

• Yokoyama Keiichi

  Department Of Pathology And Cell Regulation Kyoto Prefectural University Of Medicine

• Yokoyama Keiichi

  Department Of Biotechnology Graduate School Of Agricultre And Life Science The University Of Tokyo

• Yokoyama Keiichi

  Food Research & Development Laboratories

• Igarashi Yasuo

  Department Of Agricultural Chemistry The University Of Tokyo

• Ono T

  Iwate Univ. Morioka Jpn

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• Yokoyama K

  Ajinomoto Co. Inc. Kawasaki Jpn

• YOKOYAMA Keiichi

  Institute of Life Sciences, Ajinomoto Co., Inc.

• Yokoyama K

  Central Research Laboratories Ajinomoto Co. Inc.

• Ono Tetsuya

  Department Of Cell Biology Graduate School Of Medicine Tohoku University

• Yokoyama Keiichi

  京都府立医科大学 第2病理

• Kodama Tohru

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Yokoyama Keiichi

  Institute Of Life Sciences Ajinomoto Co. Inc.

• Ono Tetsuya

  Department of Biotechnology, The University of Tokyo

• ISHII Masaharu

  Department of Agricultural Chemistry, The University of Tokyo

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