Cloning, Nucleotide Sequence, and Characterization of the Genes Encoding Enzymes Involved in the Degradation of Cumene to 2-Hydroxy-6-Oxo-7-Methylocta-2,4-Dienoic Acid in Pseudomonas fluorescens IP01

スポンサーリンク

概要

• 論文の詳細を見る

• A gene cluster encoding cumene (isopropylbenzene-degrading enzymes was cloned in Escherichia coli JM109 from newly isolated Pseudomonas fluorescens strain IP01. E. coli cells containing pIP103 (10.8-kb insert) converted cumene, orther monocyclic aromatic hydrocarbons and biphenyl into the meta-cleaved compounds, whereas the cells containing pIP107D (4.8-kb insert) formed the cis-dihydrodiol compounds, and the cells containing pIP107 (5.2-kb insert) formed the catechol derivatives. We proved that this oxidation system degrades cumene using three enzymes, aromatic-ring dioxygenase, dihydrodiol dehydrogenase, and extradiol dioxygenase. Moreover we determined the nucleotide sequence of a 6,508-bp region encoding aromatic-ring dioxygenase, dihydrodiol dehydrogenase, and extradiol dioxygenase, which subsequently led to identification of six out of seven open reading frames (ORFs) from P. fluorescens IP01. The nucleotide and deduced amino acid sequences for ORF1,2,4 and 5,designated cumA1A2A3A4,were found to be similar to those for known multicomponent dioxygenase systems : todC1C2BA from Pseudomonas putida F1 (51-65% amino acid sequence identity) and bphA1A2A3A4 from Pseudomonas pseudoalcaligenes KF707 (59-78%). Two Cys-His pairs in the large subunit of iron-sulfur proteins were conserved in CumA1,and a similar Cys-His pair, conserved in the ferredoxin component of other dioxygenase systems, was found in CumA3. ORF6,designated cumB, was homologous to the dihydrodiol dehydrogenase gene of the tod (58%) and bph (73%) systems, while ORF7,designated cumC, was homologous to todE (47%) and bphC (57%) which encode the meta-cleavage enzymes. Analysis of strain IP01's nucleotide sequence revealed an overall G+C content of 53% for the cum gene cluster, less than the general G+C content of the chromosome of P. fluorescens.

• 社団法人日本生物工学会の論文
• 1996-03-25

著者

• KODAMA Tohru

  Department of Textile Science and Technology, Shinshu University

• YAMANE Hisakazu

  Biotechnology Research Center, The University of Tokyo

• OMORI TOSHIO

  Biotechnology Research Center, The University of Tokyo

• Omori T

  Coll. Of Systems Engineering And Sci. Dep. Of Bioscience And Engineering Shibaura Inst. Of Technol.

• HABE HIROSHI

  Biotechnology Research Center, The University of Tokyo

• KIMURA TOSHIAKI

  Biotechnology Research Center, The University of Tokyo

• AOKI HIROBUMI

  Biochemistry Department, Central Research Laboratory, Showa Denko Co. Ltd.,

• Omori Toshio

  Biotechnoiogy Researchcenter The University Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center The Univ. Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center Univ. Of Tokyo

• Yamane Hisakazu

  Biotech. Res. Ctr. The Univ. Of Tokyo

• Habe Hiroshi

  Biotechnology Research Center University Of Tokyo

• Kodama T

  Department Of Biotechnology The University Of Tokyo:(present Address)faculty Of Textile Science And

• Kodama Tohru

  Department Of Agricultural Chemistry The University Of Tokyo

• Kokubo Tetsuro

  Department Of Biotechnology The University Of Tokyo:(present Address)division Of Gene Function In An

• Kimura Toshiaki

  Biotechnology Development Department Of Production Shionogi & Co. Ltd.

• Habe H

  Research Institute For Innovations In Sustainable Chemistry National Institute Of Advanced Industria

• Kodama Tohru

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Aoki H

  Biochemistry Department Central Research Laboratory Showa Denko Co. Ltd.

• Yada H

  Biotechnology Research Center University Of Tokyo

• YAMANE Hisakazu

  Biotech. Res. Ctr. , The Univ. of Tokyo

関連論文

• Carboxylation Reactions of Pyruvate:Ferredoxin Oxidoreductase and 2-Oxoglutarate:Ferredoxin Oxidoreductase from Hydrogenobacter thermophilus TK-6
• Purification and Characterization of Ferredoxin from Hydrogenobacter thermophilus Strain TK-6
• Purification and Some Properties of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from a Thermophilic Hydrogen-Oxidizing Bacterium, Pseudomonas hydrogeneothermophila Strain TH-1
• Methionaquinone is a Direct Natural Electron Acceptor for the Membrane-bound Hydrogenase in Hydrogenobacter thermophilus Strain TK-6(Biological Chemistry)
• Colony Formation of Hydrogenobacter thermophilus on a Plate Solidified with GELRITE(Microbiology & Fermentation Industry)
• Purification and Some Properties of Cytochrome c552 from Hydrogenobacter thermophilus
• The Membrane-bound Hydrogenase Reduces Cytochrome c552 in Hydrogenobacter thermophilus strain TK-6
• A Series of Crystal Structures of a meta-Cleavage Product Hydrolase from Pseudomonas fluorescens IP01 (CumD) Complexed with Various Cleavage Products
• Purification, Characterization, and Steady-State Kinetics of a meta-Cleavage Compound Hydrolase from Pseudomonas fluorescens IP01
• Expression Pattern of the CsPK3 Auxin-Responsive Protein Kinase Gene
• Detection of a Bacterial Group within the Phylum Chloroflexi and Reductive-Dehalogenase-Homologous Genes in Pentachlorobenzene-Dechlorinating Estuarine Sediment from the Arakawa River, Japan
• Isolation and Characterization of an Alkaliphilic Bacterium Utilizing Pyrene as a Carbon Source(ENVIRONMENTAL BIOTECHNOLOGY)
• Purification and Characterization of meta-Cleavage Compound Hydrolase from a Carbazole Degrader Pseudomonas resinovorans Strain CA10(Microbiology & Fermentation Technology)
• Evidence for the Presence of DNA-Binding Proteins Involved in Regulation of the Gene Expression of Indole-3-Pyruvic Acid Decarboxylase, a Key Enzyme in Indole-3-Acetic Acid Biosynthesis in Azospirillum lipoferum FS
• New Classification System for Oxygenase Components Involved in Ring-Hydroxylating Oxygenations
• Isolation and Characterization of Low-indole-3-acetic Acid-producing Mutants from Bradyrhizobium elkanii
• Polypeptide Requirement of Multicomponent Monooxygenase DsoABCDEF for Dimethyl Sulfide Oxidizing Activity
• Cloning and Characterization of the Genes Involved in the Degradation of Dibenzofuran by Terrabacter sp. Strain DBF63
• Cloning and Nucleotide Sequences of the Genes Involved in the meta-Cleavage Pathywa of Cumene Degradation in Pseudomonas fluorescens IP01
• Cloning, Nucleotide Sequence, and Characterization of the Genes Encoding Enzymes Involved in the Degradation of Cumene to 2-Hydroxy-6-Oxo-7-Methylocta-2,4-Dienoic Acid in Pseudomonas fluorescens IP01
• Desulfurization of Alkyl and Aromatic Sulfides and Sulfonates by Dibenzothiophene-desulfurizing Rhodococcus sp. Strain SY1
• Diterpene Phytoalexins Are Biosynthesized in and Exuded from the Roots of Rice Seedlings
• Involvement of the Basic Helix-Loop-Helix Transcription Factor RERJ1 in Wounding and Drought Stress Responses in Rice Plants
• Preparation and Biological Activity of Molecular Probes to Identify and Analyze Jasmonic Acid-binding Proteins
• 1P-023 芳香環水酸化ジオキシゲナーゼの電子伝達コンポーネント(ferredoxin reductase)のX線結晶構造解析(蛋白質・構造(1),第46回日本生物物理学会年会)
• CLONING OF ent-KAURENE SYNTHASE B cDNA FROM PUMPKIN(Cucurbita maxima L. )
• Elicitor and Calatalse Activity of Conidia Suspensions of Various Strains of Magnaporthe grisea in Suspension-Cultured Cells of Rice
• Induction of Resistance against Rice Blast Fungus in Rice Plants Treated with a Potent Elicitor, N-Acetylchitooligosaccharide
• Characterization of an Elicitor-Induced Rice WRKY Gene, OsWRKY71
• Pael receptor is involved in dopamine metabolism in the nigrostriatal system
• Purification and Characterization of Membrane-bound Hydrogenase from a Thermophilic Hydrogen-Oxidizing Bacterium, Pseudomonas hydrogenothermophila Strain TH-1
• Isolation and Cultivation of Thermophilic Cyanobacteria from Hot Springs of Northern Thailand
• Electron Transport System in Pseudomonas hydrogenothermophila Strain TH-1, a Thermophilic Hydrogen-oxidizing Bacterium
• Diterpene Cyclases Responsible for the Biosynthesis of Phytoalexins, Momilactones A, B, and Oryzalexins A-F in Rice
• Isolation and Identification of Antheridiogens in the Ferns, Lygodium microphyllum and Lygodium reticulatum(Organic Chemistry)
• Cloning and Sequence of the L_2 Form of RubisCO from a Marine Obligately Autotrophic Hydrogen-oxidizing Bacterium, Hydrogenovibrio marinus Strain MH-110
• Production and Properties of Glycogen in the Marine Obligate Chemolithoautotroph, Hydrogenovibrio marinus
• Growth Characteristisc and High Cell-Density Cultivation of a Marine Obligately Chemolithoautotrophic Hydrogen-Oxidizing Bacterium Hydrogenovibrio marinus Strain MH-110 under a Continuous Gas-Flow System
• Thermostability of Pseudomonas hydrogenothermophila Cytochrome c-552
• Bacterial Lipopolysaccharides Induce Defense Responses Associated with Programmed Cell Death in Rice Cells
• Production of Recombinant Cytochrome c-551 in a Pseudomonas aeruginosa Mutant Strain
• Structure and ANR-Dependent Transcription of the nir Genes for Denitritication from Pseudomonas aeruginosa
• Heterologous Expression of Hydrogenobacter thermophilus Cytochrome c-552 in the Periplasm of Pseudomonas aeruginosa
• cDNA CLONING OF ent-KAURENE SYNTHETASEA FROM RICE
• Identification of Gibberellins and 9,15-Cyclogibberellins in Developing Apple Seeds
• Gibberellink and Antheridiogens in Prothallia and Sporophytes of Anemia phyllitidis
• Effects of Fluorogibberellins on Plant Growth and Gibberellin 3β-Hydroxylases
• Inhibition of ent-Kaurene Synthase by Quaternary Ammonium Growth Retardants
• Effects of Gibberellins and Prohexadione on the Activities of Oryzain and α-Amylase in Rice Seeds : PROTEINS, ENZYMES AND METABOLISM
• Characterization of a Rice Gene Family Encoding Type-A Diterpene Cyclases
• A Step in the Biosynthesis of Gibberellins that Is Controlled by the Mutation in the Semi-Dwarf Rice Cultivar Tan-Ginbozu
• Structural and Functional Characterization of Oryzacystatin-I Monomer and Dimer
• cDNA Cloning and Characterization of Gibberellin-responsive Genes in Photoblastic Lettuce Seeds
• Effects of Exogenously Applied Gibberellin and Red Light on the Endogenous Levels of Abscisic Acid in Photoblastic Lettuce Seeds : GROWTH AND DEVELOPMENT
• Promotive Effect of Auxins on UDP-Glucose : Flavonol Glucosyltransferase Activity in Vitis sp.Cell Cultures
• Quercetin 3-0-β-D-Glucopyranoside and Isorhamnetin 3-O-β-D-Glucopyranoside Formation from Quercetin by Cell Cultures of Ipomoea batatas and Crocus sativum(Biological Chemistry)
• Glucosylation of Quercetin by a Cell Suspension Culture of Vitis sp.(Biological Chemistry)
• Cell Mass Production of Acinetobacter calcoaceticus in Palm Oil-Limited Chemostant Culture
• Characterization of a Protein Kinase Gene Responsive to Auxin and Gibberellin in Cucumber Hypocotyls
• Cloning, Sequence and Overexpression of the Thermophilic Cyanobacterium Gene for the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase
• Purification of RuBisCO from the Thermophilic Cyanobacterium Synechococcus sp. Strain a-1
• Qualitative and Quantitative Analysis of Endogenous Gibberellins in Raphanus sativus L. during Cold Treatment and the Subsequent Growth
• Construction of Novel Expression Vectors Effective in Pseudomonas Cells(Microbiology & Fermentation Industry)
• Alteration of the Substrate Specificity of the Angular Dioxygenase Carbazole 1,9a-Dioxygenase
• Cloning and Expression of a Clostridium cellobioparum Cellulase Gene and Its Excretion from Escherichia coil JM109
• Acetoacetyl-CoA Transferase and Thiolase Reactions Are Catalyzed by a Single Enzyme in Clostridium acetobutylicum ATCC824(Microbiology & Fermentation Industry)
• Molecular Cloning and Sequence Analysis of the Gene Encoding an Endo _α-1,4 Polygalactosaminidase of Pseudomonas sp. 881
• Biodegradation of Carbazole by Pseudomonas spp. CA06 and CA10
• A Non-radioisotopic Anion-exchange Chromatographic Method to Measure the CO_2/O_2 Specificity Factor for Ribulose Bisphosphate Carboxylase/Oxygenase
• Effect of Carbon Cioxide Concentration on the Growth and RubisCO Activity of a Thermophilic Cyanobacterium, Chroococcidiopsis sp. Strain TS-821
• Preparation of (±)-2-(2,3-^2H_2)Jasmonic Acid and Its Methyl Ester, Methyl (±)-2-(2,3-^2H_2)Jasmonate
• Behavior of Various Hosts of the IncP-7 Carbazole-Degradative Plasmid pCAR1 in Artificial Microcosms
• The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in Pseudomonas within Natural Environments and Microcosms
• The Complete Nucleotide Sequence of pCAR2: pCAR2 and pCAR1 Were Structurally Identical IncP-7 Carbazole Degradative Plasmids
• Cloning and Characterization of cDNAs Encoding ent-Copalyl Diphosphate Synthases in Wheat: Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes
• Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
• Characterization of Chilli Pepper hairy Roots Expressing the Parsley PAL2 cDNA
• Transformation of Chilli Pepper (Capsicum frutescens) with a Phenylalanine Ammonia-Lyase Gene
• トウガラシ(Capsicum frutescens)の毛状根培養
• Mode of Action for Mitomycin C Inactivating Factors of Pseudomonas convexa 4-87(Biological Chemistry)
• Partial Purification and Some Properties of Mitomycin C Inactivating Factors of Pseudomonas convexa 4-87(Biological Chemistry)
• Genetic Evidence for the Role of Isopentenyl Diphosphate Isomerases in the Mevalonate Pathway and Plant Development in Arabidopsis
• Release of neurotransmitters in the monkey frontal cortex is related to level of attention
• 4-O-Caffeoylshikimic and 4-O-(p-Coumaroyl)shikimic Acids from the Dwarf Tree Fern, Dicksonia antarctica
• Bioactive Gibberellin and Its Metabolism in Shoots of Phaseolus vulgaris
• Involvement of Gibberellins in Expression of a Cysteine Proteinase (SH-EP) in Cotyledons of Vigna mungo Seedlings
• POSSIBLE INVOL VEMENT OF GIBBERELLINS IN EXPRESSION OF PROTEINASE ACTIVITY IN COTYLEDONS OF Vigna mungo SEEDLINGS
• Effects of Salicylic Acid on the Production of Procyanidin and Anthocyanin in Cultured Grape Cells
• Microbial Degradation of the Polycyclic Aromatic Hydrocarbons Acenaphthene and Acenaphthylene by a Pure Bacterial Culture
• Promoter Analysis of the Elicitor-Induced WRKY Gene OsWRKY53, Which Is Involved in Defense Responses in Rice
• Alteration of the Substrate Specificity of the Angular Dioxygenase Carbazole 1,9a-Dioxygenase
• Relative power contributions of unit discharges simultaneously recorded in the mesencephalic reticular formation
• Acetylcholine and glutamate release during sleep-wakefulness in the pedunculopontine tegmental nucleus and norepinephrine changes regulated by nitric oxide
• Elicitor and Calatalse Activity of Conidia Suspensions of Various Strains of Magnaporthe grisea in Suspension-Cultured Cells of Rice
• Diterpene Phytoalexins Are Biosynthesized in and Exuded from the Roots of Rice Seedlings
• Comparison of the Enzymatic Properties of ent-Copalyl Diphosphate Synthases in the Biosynthesis of Phytoalexins and Gibberellins in Rice
• cDNA Cloning and Characterization of a Gibberellin-Responsive Gene in Hypocotyls of Cucumis sativus L.
• Cloning and Characterization of cDNAs Encoding ent-Copalyl Diphosphate Synthases in Wheat : Insight into the Evolution of Rice Phytoalexin Biosynthetic Genes
• Characterization of an Elicitor-Induced Rice WRKY Gene, OsWRKY71
• Identification of the Electron Transfer Flavoprotein as an Upregulated Enzyme in the Benzoate Utilization of Desulfotignum balticum

もっと見る 閉じる

スポンサーリンク