Polypeptide Requirement of Multicomponent Monooxygenase DsoABCDEF for Dimethyl Sulfide Oxidizing Activity
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概要
- 論文の詳細を見る
In our previous study, the multicomponent monooxygenase DsoABCDEF in Acinetobacter sp. strain 20B was cloned based on its ability to oxidize dimethyl sulfide (DMS) to dimethyl sulfoxide (DMSO) in E. coli cells, which had high sequence similarity with phenol hydroxylase MopKLMNOP in Acinetobacter calcoaceticus NCIB8250,DmpKLMNOP in Pseudomonas sp. CF600 and some other multicomponent monooxygenases. In this study, DsoB, C, D, E, and F were found to be needed for DMS-oxidizing activity in polypeptide requirement experiments, while DsoA was not necessary for it. It was also found that complementation of the deletion mutants lacking DsoC or F with the corresponding Dmp polypeptides supported the DMS-oxidizing activity, while complementation of the deletion mutants lacking any of the oxygenase subunits (DsoB, D, or E) with the corresponding Dmp polypeptides reduced or nullified the activity.
- 社団法人日本農芸化学会の論文
- 1999-10-23
著者
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Horinouchi Masae
Biotechnology Research Center The University Of Tokyo:(present Address)laboratory Of Microbiology Th
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Horinouchi Masae
Biotechnology Research Center The University Of Tokyo
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吉田 豊和
岐阜大・工・生命工
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NOJIRI Hideaki
Biotechnology Research Center, The University of Tokyo
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YAMANE Hisakazu
Biotechnology Research Center, The University of Tokyo
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OMORI TOSHIO
Biotechnology Research Center, The University of Tokyo
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Omori T
Coll. Of Systems Engineering And Sci. Dep. Of Bioscience And Engineering Shibaura Inst. Of Technol.
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YOSHIDA Takako
Biotechnology Research Center, The University of Tokyo
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Omori Toshio
Biotechnoiogy Researchcenter The University Of Tokyo
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Nojiri H
Biotechnology Res. Center The Univ. Of Tokyo
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Noguchi Haruko
Biotechnology Research Center The University Of Tokyo
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Yamane Hisakazu
Biotechnology Res. Center The Univ. Of Tokyo
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Yamane Hisakazu
Biotechnology Res. Center Univ. Of Tokyo
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Yamane Hisakazu
Biotech. Res. Ctr. The Univ. Of Tokyo
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Nojiri Hideaki
Biotechnology Res. Center The Univ. Of Tokyo
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Yada H
Biotechnology Research Center University Of Tokyo
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Omori Toshio
Shibaura Institute Of Technology
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YAMANE Hisakazu
Biotech. Res. Ctr. , The Univ. of Tokyo
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