Characterization of a Protein Kinase Gene Responsive to Auxin and Gibberellin in Cucumber Hypocotyls

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概要

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• By means of the PCR, cDNA clones encoding putative protein kinases have been obtained from cucumber hypocotyls. The abundance of the transcript of one of these genes, which was named CsPK3, increased on treatment with gibberellin(GA_4) and/or auxin(IAA). We screened a cucumber cDNA library to clone CsPK3 cDNA. The cDNA clone(cCsPK3) encodes an open reading frame of 1, 413 bp(471 amino acids), and its predicted amino acid sequence showed homology with those of serine/threonine protein kinases. Northern blot analysis indicated that IAA was more active than GA_4 in increasing the level of CsPK3 mRNA in cucumber hypocotyls and that the increase in the level of CsPK3 mRNA on treatment with IAA was not inhibited by pretreatment with a protein synthesis inhibitor. The level of CsPK3 mRNA was high in hypocotyls of dark-grown cucumber seedlings and decreased to less than 50% of the original level within 15 min of the start of irradiation with white light.

• 日本植物生理学会の論文

著者

• YAMANE Hisakazu

  Biotechnology Research Center, The University of Tokyo

• NEMOTO Keisuke

  Asian Natural Environmental Science Center, The University of Tokyo

• MUROFUSHI Noboru

  Faculty of Bioresource Sciences, Akita Prefectural University

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, The University of Tokyo

• MUROFUSHI Noboru

  Department of Biotechnology, Akita Prefectural University

• Chono Makiko

  Department Of Wheat And Barley Research National Institute Of Crop Science

• Chono Makiko

  National Institute Of Crop Science (nics)

• Maejima Noboru

  Department Of Biotechnology Akita Prefectural University

• Nemoto K

  Asian Natural Environmental Science Center The University Of Tokyo

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center The Univ. Of Tokyo

• Yamane Hisakazu

  Biotechnology Res. Center Univ. Of Tokyo

• Yamane Hisakazu

  Biotech. Res. Ctr. The Univ. Of Tokyo

• Murofushi Noboru

  Faculty Of Bioresource Sciences Akita Prefectural University

• Murofushi Noboru

  Department Of Agricultural Chemistry The University Of Tokyo

• Yamaguchi Isomaro

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• Chono Makiko

  Bio-oriented Technology Research Advancement Institution(BRAIN)

• Nemoto Keisuke

  Department of Agricultural and Environmental Biology, The University of Tokyo

• Yada H

  Biotechnology Research Center University Of Tokyo

• Chono Makiko

  Naro Institute Of Crop Science

• Yamaguchi Isomaro

  Dept. Appl. Biol. Chem. Tokyo Univ.

• YAMANE Hisakazu

  Biotech. Res. Ctr. , The Univ. of Tokyo

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