Expression of a Single-Chain Antibody against GA_<24/19> in Vascular Tissues Induces Dwarf Phenotype for Rice Plants(Plant Nutrition)

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概要

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• GA_1 and GA_4 are active gibberellins (GAs) in rice plants, which are synthesized from GA_<19> and GA_<24>, respectively. However, the plant tissues where active GAs biosynthesis occurs and the route of their transport have not been fully elucidated. To study the importance of vascular tissues for the function of GAs, cDNA encoding anti-GA_<24/19> single-chain variable fragment (scFv) was expressed under the control of the thioredoxin h promoter, which is active in companion cells of phloem tissues. The transgenic rice plants showed a dwarf phenotype with increased levels of GA_<19>. This indicated that the expression of the anti-GA_<24/19> scFv antibody in companion cells changed the synthesis and/or translocation of GAs in rice plants effectively, suggesting that the vascular tissues play an important role in the function of GAs in rice plants.

• 社団法人日本土壌肥料学会の論文

著者

• HAYASHI Hiroaki

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• YONEYAMA Tadakatsu

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• Hayashi Hiroaki

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• FUJIWARA Toru

  Department of Applied Biological Chemistry, The University of Tokyo

• Fujiwara T

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Fujiwara Toru

  Osaka University

• Fukuda Akari

  Dept. Appl. Biol. Chem. Univ. Tokyo

• TANAKA Yoko

  Department of Pediatrics, Gifu University School of Medicine

• Hayashi H

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• Hayashi H

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• SUZUKI Yoshihito

  Department of Applied Biological Chemistry, The University of Tokyo

• YAMAGUCHI Isomaro

  Department of Applied Biological Chemistry, The University of Tokyo

• FUKUDA Akari

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• Nagai Seiji

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• Fujiwara T

  Osaka University

• Fujiwara Toru

  Graduate School Of Engineering Science Osaka University

• Hayashi Hiroaki

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yoneyama T

  Univ. Tokyo Tokyo

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry The University Of Tokyo

• Fujiwara Toru

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yoshida Shigeo

  Plant Functions Lab. The Inst. Of Phys. And Chem. Res. (riken)

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry The University Of Tokyo

• Yamaguchi Isomaro

  Department Of Applied Biological Chemistry University Of Tokyo

• Yamaguchi Isomaro

  Department Of Agricultural Chemistry Faculty Of Agricultural Science University Of Tokyo

• Yoneyama Tadakatsu

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yoneyama Tadakatsu

  Department Of Agricultural Chemistry University Of Tokyo:(present Address)division Of Environmental

• Yoneyama Tadakatsu

  Department Of Applied Biological Chemistry University Of Tokyo

• Yamaguchi I

  Department Of Applied Biological Chemistry University Of Tokyo

• YAMAGUCHI Tomoya

  Tohoku Natl. Agri. Exp. St.

• Nagai Seiji

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Fujiwara Toru

  Dept. Appl. Biol. Chem. Univ. Tokyo:presto Jst

• FUJIWARA Toru

  Precursory Research for Embryonic Science and Technology

• Yamaguchi I

  The Institute Of Physical And Chemical Research (riken)

• Tanaka Y

  Tokyo Dental Coll. Ichikawa Jpn

• Tanaka Yoko

  Department Of Applied Science For Electronics And Materials Graduate School Of Engineering Sciences

• Hayashi H

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Hayashi Hiroaki

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Science The Univ

• Hayashi Hiroaki

  Department Of Agricultural Cheinistry Faculty Of Agriculture University Of Tokyo

• Tanaka Yoko

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Suzuki Yoshihito

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yoneyama Tadakatsu

  Univ. Tokyo Tokyo

• Hayashi Hiroaki

  Department Of Applied Biological Chemistry The University Of Tokyo

• Yamaguchi Isomaro

  Dept. Appl. Biol. Chem. Tokyo Univ.

• Yoneyama Tadakatsu

  Department Of Agricultural Chemistry University Of Tokyo

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