Effect of cadmium on the chemical composition of xylem exudate from oilseed rape plants (Brassica napus L.)(Plant Nutrition)

概要

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Phytoremediation is a good technique for removing cadmium (Cd) from farmland soils. To remove Cd from these soils effectively, it is necessary for Cd ions to be transported to the shoot organs for later harvest. However, the mechanism of Cd translocation to shoot organs via xylem vessels has not yet been elucidated. We selected oilseed rape plants (Brassica napus L.) and established a method to collect xylem exudates from these plants. After 3 days of Cd treatment (10μmol L^<-1> and 30μmol L^<-1>) the Cd concentrations in the xylem exudates were approximately 6.5μmol L^<-1> and 16μmol L^<-1>, respectively. The detection of Cd in the xylem exudate indicated that Cd was moving to shoot organs via xylem vessels. The effect of these Cd treatments on the amino acid, organic acid and protein composition of xylem exudates from oilseed rape plants was investigated. The level of amino acids and organic acids detected was enough to bind Cd transported via the xylem. Sodium dodecylsulfate-polyacrylamide gel electrophoresis analysis revealed that proteins with molecular weights of 36kDa and 45kDa clearly increased in the exudates with Cd treatment. The possibility that these compounds are binding Cd in the xylem exudates was discussed.
社団法人日本土壌肥料学会の論文