Amino acid metabolism in plant leaf IV. The effect of light on ammonium assimilation and glutamine metabolism in the cells isolated from spinach leaves
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概要
- 論文の詳細を見る
Comparison of incorporation of ^<15>N-labeled ammonium into amino acids in cells isolated from spinach leaves showed that ammonium was most actively incorporated into the amido-group of glutamine. The ^<15>N contents of other amino acids were less than one-tenth that of the amido-group of glutamine. L-Methionine-DL-sulfoximine (MS) suppressed the incorporation of ammonium not only into the amido-group of glutamine, but also into glutamic acid. Turning off the light after 1 min illumination increased the ^<15>N content of glutamine while it decreased that of the glutamic acid, aspartic acid and alanine. Illumination of the cells after the application of ammonium had a more significant effect on ammonium assimilation than illumination before the application of ammonium. When ^<14>C-U-^<15>N (amido labeled)-glutamine was added to the cell suspension, the transfer of amido-group of glutamine was completely inhibited in the dark, but no difference in the flow of ^<14>C was observed. These results suggest that glutamine synthetase (GS) and glutamate synthase (GO-GAT) pathways operate in ammonium assimilation in the cells isolated from spinach leaves, and that the former is light-independent but the latter is light-dependent.
- 日本植物生理学会の論文
著者
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ITO Osamu
Department of Neurosurgery, Shi-Koga Hospital
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YONEYAMA Tadakatsu
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Yoneyama Tadakatsu
Department Of Agricultural Chemistry University Of Tokyo:(present Address)division Of Environmental
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Kumazawa Kikuo
Department Of Agricultural Chemistry Univerisy Of Tokyo
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Kumazawa Kikuo
Department Of Agricultural Chemistry University Of Tokyo
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Kumazawa Kikuo
Department Of Agricultural Chemistry The University Of Tokyo
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Ito Osamu
Department Of Agricultural Chemistry University Of Tokyo:(present Address)department Of Soil Microbi
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Ito Osamu
Department Of Agricultural Chemistry University Of Tokyo
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Yoneyama Tadakatsu
Department Of Agricultural Chemistry University Of Tokyo
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