Evidence of ammonium assimilation via the glutamine synthetase-glutamate synthase system in rice seedling roots

概要

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When rice seedling roots were fed ^<15>N-ammonium for 1 hr, the amide nitrogen of glutamine showed the highest ^<15>N abundance. Moreover, glutamine amino, glutamic acid, aspartic acid and alanine showed higher ^<15>N abundance than ammonium did. In roots whose GS activity was inhibited with MS, both the amount of ammonium and its ^<15>N abundance were increased. In contrast, both the amount of all examined amino acids containing glutamic acid and their ^<15>N abundance decreased in roots whose GS activity was inhibited. From these results, it could be concluded that the first step of ammonium assimilation in rice seedling roots was mainly glutamine synthesis by GS and the second was glutamic acid formation by the GOGAT system. The results of an experiment using ^<15>N-labelled glutamine also supported this conclusion.
日本植物生理学会の論文