Cytosolic glutamine synthetase is present in the phloem sap of rice (Oryza sativa L.)(Plant Nutrition)

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概要

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• The presence of glutamine synthetase (GS) in the rice sieve tube was examined. Proteins in the rice phloem sap from leaf sheaths were separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis, transferred to polyvinylidine difluoride membranes and immunoblotted with anti-GS1 antibody. A cross-reacting band, thought to be GS1, was detected in the phloem sap. Moreover, the phloem sap contained a significant amount of GS transferase activity. Previous studies have shown that the concentrations of substrates and cofactors in the rice phloem sap are sufficient for cytosolic GS reaction. These data suggest that physiologically active GS1 is present in rice phloem sap, which might convert glutamate to glutamine in vivo.

著者

• TANAKA Tomohiro

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• SUZUI Nobuo

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• HAYASHI Hiroaki

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• YAMAYA Tomoyuki

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University

• YONEYAMA Tadakatsu

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• Hayashi Hiroaki

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• Tanaka Tomohiro

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Tanaka Tomohiro

  Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University

• Hayashi H

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• Hayashi H

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Suzui N

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Suzui Nobuo

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yamaya Tomoyuki

  Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University

• Yamaya T

  Tohoku Univ. Sendai Jpn

• Yamaya T

  Institute For Agricultural And Biological Sciences. Okayama University

• Yamaya Tomoyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Yamaya Tomoyuki

  Dept. Applied Plant Sci. Graduate School Of Agric. Sci. Tohoku Univ.

• Suzui Nobuo

  Univ. Tokyo Tokyo

• Hayashi Hiroaki

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yoneyama T

  Univ. Tokyo Tokyo

• Yamaya Tomoyuki

  Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G

• Yamaya Tomoyuki

  Department Of Applies Plant Science Graduate School Of Agricultural Science Tohoku University

• Yoneyama Tadakatsu

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Yoneyama Tadakatsu

  Department Of Agricultural Chemistry University Of Tokyo:(present Address)division Of Environmental

• Yoneyama Tadakatsu

  Department Of Applied Biological Chemistry University Of Tokyo

• YAMAGUCHI Tomoya

  Tohoku Natl. Agri. Exp. St.

• Hayashi H

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Hayashi Hiroaki

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Science The Univ

• Yamaya Tomoyuki

  Department Of Agricultural Cheimistry Faculty Of Agriculture Tohoku University

• Hayashi Hiroaki

  Department Of Agricultural Cheinistry Faculty Of Agriculture University Of Tokyo

• Yoneyama Tadakatsu

  Univ. Tokyo Tokyo

• Hayashi Hiroaki

  Department Of Applied Biological Chemistry The University Of Tokyo

• Yoneyama Tadakatsu

  Department Of Agricultural Chemistry University Of Tokyo

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