Expressed nifH Genes of Endophytic Bacteria Detected in Field-Grown Sweet Potatoes-Ipomoea batatas L.
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概要
- 論文の詳細を見る
We examined the nitrogenase reductase (nifH) genes of endophytic diazotrophic bacteria expressed in field-grown sweet potatoes (Ipomoea batatas L.) by reverse transcription (RT)-PCR. Gene fragments corresponding to nifH were amplified from mRNA obtained from the stems and storage roots of field-grown sweet potatoes several months after planting. Sequence analysis revealed that these clones were homologous to the nifH sequences of Bradyrhizobium, Pelomonas, and Bacillus sp. in the DNA database. Investigation of the nifH genes amplified from the genomic DNA extracted from these sweet potatoes also showed high similarity to various α-proteobacteria including Bradyrhizobium, β-proteobacteria, and cyanobacteria. These results suggest that bradyrhizobia colonize and express nifH genes not only in the root nodules of leguminous plants but also in sweet potatoes as diazotrophic endophytes.
- 日本微生物生態学会の論文
著者
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YONEYAMA Tadakatsu
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Yoneyama T
Univ. Tokyo Tokyo
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Ohwaki Yoshinari
National Agricultural Research Center (NARC)
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Yamakawa Hiromoto
National Agricultural Research Center
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Yamakawa Hiromoto
National Agricultural Research Center Hokuriku Research Center
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Yoneyama Tadakatsu
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Yoneyama Tadakatsu
Department Of Agricultural Chemistry University Of Tokyo:(present Address)division Of Environmental
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Yoneyama Tadakatsu
Department Of Applied Biological Chemistry University Of Tokyo
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YAMAGUCHI Tomoya
Tohoku Natl. Agri. Exp. St.
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Fujihara Shinsuke
National Agricultural Research Center
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Terakado-tonooka Junko
National Agricultural Research Center:jsps Research Fellow Japan Society For The Promotion Of Scienc
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Yamakawa Hiromoto
National Agricultural Res. Center Niigata Jpn
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TANAKA FUKUYO
National Agricultural Research Center
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Fujihara S
National Agricultural Research Center
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Ohwaki Y
National Agricultural Research Center
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Ohwaki Yoshinari
National Agricultural Research Center
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Yoneyama Tadakatsu
Univ. Tokyo Tokyo
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Yoneyama Tadakatsu
Department Of Agricultural Chemistry University Of Tokyo
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Terakado-Tonooka Junko
National Agricultural Research Center.
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