Effects of Oxygen Concentration and Leghemoglobin on Organic Acid Degradation by Isolated Soybean Nodule Bacteriods
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概要
- 論文の詳細を見る
Bacteroids retaining high acetylene reduction activity (nitrogenase activity) were prepared anaerobically from soybean nodules. Addition of succinate (or of both leghemoglobin and succinate) to the acetylene reduction assay system greatly increased the activity of the isolated bacteroids. When various organic acids were incubated with the bacteroids at 2% oxygen concentration, an optimum condition for bacteroid acetylene reduction, the organic acid degradation by bacteroids was very slow, and both lactate and acetate were accumulated in the incubation system, suggesting the operation of fermentative pathway in bacteroids under such low oxygen conditions. With 20% oxygen, the added organic acids were degraded rapidly by bacteroids without addition of leghemoglobin to the incubation system. With leghemoglobin in the incubation system, the organic acid degradation by bacteroids was accelerated extensively even at 2% oxygen, and the formation of lactate and acetate were negligible. No significant difference in the organic acid degradation rate was observed between the 2% and 20% oxygen concentrations when the leghemoglobin was present in the incubation system. Addition of acetylene to the assay system slightly inhibited the organic acid degradation. This data suggests that bacteroids are unable to oxidize organic acid in low oxygen concentration and that the leghemoglobin allows the rapid organic acid dagradation by bacteroids even in such low oxygen concentrations.
- 社団法人 日本農芸化学会の論文
著者
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Tajima Shigeyuki
Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University
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Ishizuka Junji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Kouchi Hiroshi
Department Of Agricultural Chemistry University Of Tokyo
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Yoneyama Tadakatsu
Department Of Agricultural Chemistry University Of Tokyo
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Kouchi Hiroshi
Department of Applied Physiology, National Institute of Agrobiological Resources
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SASAHARA Hiroyuki
The Kagawa Prefectural Fermentation & Foods Experimental Station Takamatsu Branch
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