Involvement of a Novel Genistein-Inducible Multidrug Efflux Pump of Bradyrhizobium japonicum Early in the Interaction with Glycine max (L.) Merr

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概要

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• The early molecular dialogue between soybean and the bacterium Bradyrhizobium japonicum is crucial for triggering their symbiotic interaction. Here we found a single large genomic locus that is widely separated from the symbiosis island and was conspicuously induced within minutes after the addition of genistein. This locus (named BjG30) contains genes for the multidrug efflux pump, TetR family transcriptional regulator, and polyhydroxybutyrate (PHB) metabolism. The induction of BjG30 by genistein was competitively inhibited by daidzein, although both genistein and daidzein are soybean-derived inducers of nodulation (nod) genes. Such a differential expression pattern is also observed in some legume-derived flavonoids, which structurally differ in the hydroxy/deoxy group at the 5-position. In addition, not only did the induction start far in advance of nodW and nodD1 after the addition of genistein, but the levels showed distinct concentration dependence, indicating that the induction pattern of BjG30 is completely different from that of nod genes. The deletion of genes encoding either the multidrug efflux pump or PHB metabolism, especially the former, resulted in defective nodulation performance and nitrogen-fixing capability. Taken together, these results indicate that BjG30, and especially its multidrug efflux pump, may play a key role in the early stage of symbiosis by balancing the dual functions of genistein as both a nod gene inducer and toxicant.

• 日本微生物生態学会の論文

著者

• UCHIUMI TOSHIKI

  Graduate School of Science and Engineering, Kagoshima University

• ABE MIKIKO

  Graduate School of Science and Engineering, Kagoshima University

• Saeki Kazuhiko

  Department Of Biological Sciences Faculty Of Science Nara Women's University

• Ohwada Takuji

  Department Of Agricultural And Life Sciences Obihiro University Of Agriculture And Veterinary Medici

• Tajima Shigeyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University

• Uchiumi Toshiki

  Graduate School Of Sci. And Engineering Kagoshima Univ.

• Minamisawa Kiwamu

  Graduate School Of Life Sciences Tohoku Univ.

• Tabata Satoshi

  Kazusa Dna Institute

• Mitsui Hisayuki

  Graduate School Of Life Science Tohoku University

• Itakura Manabu

  Graduate School Of Life Science Tohoku University

• Tokuji Yoshihiko

  Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine

• MITSUI HISAYUKI

  Graduate School of Life Science, Tohoku University

• Oomori Hirofumi

  Graduate School of Science, Osaka University

• Itakura Manabu

  Graduate School of Life Science, Tohoku University

• Takeshima Keisuke

  Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine

• Hidaka Tatsuo

  Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine

• Wei Min

  School of Life Science, Lanzhou University

• Yokoyama Tadashi

  Graduate School of Agriculture, Tokyo University of Agriculture and Technology

• Kaneko Takakazu

  Faculty of Engineering, Kyoto Sangyo University

• Tajima Shigeyuki

  Department of Life Science, Kagawa University

• Minamisawa Kiwamu

  Graduate School of Life Science, Tohoku University

• Saeki Kazuhiko

  Department of Biological Science, Faculty of Science, Nara Women's University

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