Role of Glutathione in the Accumulation of Potassium Ions in Escherichia coli under Osmotic Stress

概要

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When Escherichia coli cells were stressed by NaCl, cellular levels of glutathione (GSH) increased, and potassium ions (K^+) accumulated. To identify the role of the stress-induced synthesis of GSH in the accumulation of K^+ a GSH-deficient mutant of E.coli was used. The mutant grew even in a medium with a low concentration of K^+ after a lag of about 32 hr. In such cells grown under osmotic stress in this medium, K^+ did not accumulate in the lag phase. In the logarithmic phase, however, intracellular levels of K^+ increased in spite of the lack of intracellular GSH. In a medium with the usual K^+ concentration, K^+ accumulated in a pattern similar to that of the parent strain in both phases. These results showed that GSH participates in the accumulation of K^+ in cells under osmotic stress only in the lag phase in medium with little K^+.
社団法人日本農芸化学会の論文
1992-04-23