Cadaverine Involved in Urate Degrading Activity (Uricase Activity) in Soybean Radicles

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Cadaverine, a 5-carbon diamine, was identified as the cofactor of uricase activity previously found in soybean seedlings. The substance purified from freeze dried hypocotyls was subjected to liquid chromatography, mass spectrometry, ^1H- and ^<13>C-nuclear magnetic resonance spectrometry for identification. The concentrations of cadaverine in 3-day-old radicles and hypocotyls were 2.37 mM and 5.09 mM, respectively. Other polyamine concentrations were low. Biogenic polyamines (cadaverine, putrescine, spermidine and spermine) functioned as cofactors, whereas conjugated polyamines (tyramine and histamine) and amino acids had no effect. The addition of catalase to the assay system counteracted the effect of cadaverine. Peroxide at appropriate concentrations acted like cadaverine with an identical K_m value, suggesting that urate degrading activity can be ascribed to the diamine oxidase-peroxidase system.
日本植物生理学会の論文