Possible Involvement of Optimally Phosphorylated L-Plastin in Activation of Superoxide-Generating NADPH Oxidase
スポンサーリンク
概要
- 論文の詳細を見る
The involvement of protein phosphatases in the activation of superoxide (O2-)- generating enzyme in human neutrophils was examined using calyculin A, an inhibitor of protein phosphatase type 1 and 2 A. Calyculin A inhibited the phorbol myristate acetate (PMA)- and opsonized zymosan (OZ)-activated O2- generation by human neutrophils. This inhibitory effect of calyculin A on PMA-activated O2- generation was reversed by the addition of KT5926, a specific inhibitor of myosin light chain kinase and Ca2+/calmodulin-dependent protein kinase II. These results suggest that the addition of calyculin A may cause hyperphosphorylation of some protein(s) that plays a crucial role in the PMA-dependent activation of O2- generating enzyme, and that this protein hyperphosphorylation may be evoked by a KT5926-sensitive kinase or its downstream kinase. Whereas two-dimensional analysis involving 32P revealed that calyculin A caused the hyperphosphorylation of many proteins, KT5926 mainly reduced the calyculin A-induced hyperphosphorylation of a 67 kDa protein in activated neutrophils, suggesting that the hyperphosphorylation of the 67 kDa protein might inhibit the PMA-dependent activation of NADPH oxidase. The 67 kDa cytosolic protein was moderately phosphorylated on the addition of PMA. On the other hand, in the absence of calyculin A, KT5926 inhibited both PMA-induced O2- generation and phosphorylation of the 67 kDa protein. Amino acid sequence analysis of peptides derived from the 67 kDa protein revealed that the 67 kDa protein was identical to L-plastin, an actin-bundling protein. We conclude that optimally phosphorylated L-plastin may play some crucial role in the activation of NADPH oxidase.
- 社団法人 日本生化学会の論文
著者
-
HAYAKAWA Takao
National Institute of Health Sciences
-
OSHIZAWA Tadashi
Division of Cellular and Gene Therapy Products National Institute of Health Sciences
-
Yamamoto Yukio
Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University
-
Suzuki Kazuhiro
Division Of Cellular And Gene Therapy Products National Institute Of Health Sciences
-
Yamaguchi Teruhide
Division of Biological Chemistry & Biologicals, National Institute of Health Sciences
関連論文
- Anti-tumor Responses Induced by Chemokine CCL19 Transfected into an Ovarian Carcinoma Model via Fiber-Mutant Adenovirus Vector(Biopharmacy)
- Unilateral Moyamoya Disease Associated with Multiple Aneurysms : A Case Report and Review of the Literature
- Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva
- Fruit and Vegetable Consumption and the Risk of Hypertension Determined by Self Measurement of Blood Pressure at Home : The Ohasama Study
- The Novel Compounds That Activate Farnesoid X Receptor : the Diversity of Their Effects on Gene Expression
- Neutralizing Antibody Evasion Ability of Adenovirus Vector Induced by the Bioconjugation of Methoxypolyethylene Glycol Succinimidyl Propionate(MPEG-SPA)(Biopharmacy)
- Tumor Necrosis Factor α-Gene Therapy for an Established Murine Melanoma Using RGD (Arg-Gly-Asp) Fiber-mutant Adenovirus Vectors
- Antiviral Activity of 2-Amino-4, 4α-Dihydro-4α-7-Dimethyl-3H-Phenoxazine-3-One on Poliovirus
- Emergence of Nonalbicans Candida Species Immunocompromised Leukemia Patients during Fluconazole Treatment
- Organic Acid Metabolism in Cellular Organelles of Vigna cylindrica (Mitorisasage) Cotyledons
- Suppression of Proliferation of Poliovirus and Porcine Parvovirus by Novel Phenoxazines, 2-Amino-4,4α-dihydro-4α-7-dimethyl-3H-phenoxazine-3-one and 3-Amino-1,4α-dihydro-4α-8-dimethyl-2H-phenoxazine-2-one(Pharmacology)
- Unkeito, a traditional Kampo formula, exhibits a selective estrogen receptor modulator-like activity : Prevention of osteoporosis in ovariectomized mice
- A RELATIONSHIP BETWEEN ELECTROCHEMICAL REDUCTION POTENTIAL AND THE ENZYMATIC REDUCTION OF NITRATED NAPHTHALENES BY MICROSOMES OR CYTOSOL FROM RAT LIVER
- Annexin A3 Expression Increases in Hepatocytes and is Regulated by Hepatocyte Growth Factor in Rat Liver Regeneration
- Evidences of the presence of old continental basement in Cheju volcanic Island, South Korea, revealed by radiometric ages and Nd-Sr isotopes of granitic rocks
- RESPIRATORY METABOLISM OF MITOCHONDRIA IN SOYBEAN ROOT NODULES
- CARBON METABOLISM RELATED TO NITROGEN FIXATION IN SOYBEAN ROOT NODULES
- Asymmetric Synthesis of Optically Active 3-Substituted δ-Valerolactones Using Lipase in Organic Solvents(Organic Chemistry)
- Regulation of uricase activity in developing roots of Glycine max, non-nodulating variety A62-2
- Cadaverine Involved in Urate Degrading Activity (Uricase Activity) in Soybean Radicles
- Simultaneous Measurement of Changes in the Concentration and Localization of Calcium and Actin in Migrating Neutrophils Using a Confocal Laser Scanning Microscope
- Gene Expression Profiling of Human Mesenchymal Stem Cells for Identification of Novel Markers in Early- and Late-Stage Cell Culture
- Two-dimensional electrophoretic analysis of disease-associated proteins in human cerebrospinal fluid from patients with rheumatoid arthritis
- Prostatic signet-ring cell carcinoma : Case report and literature review
- Efficient Gene Transfer into Human Trophoblast Cells with Adenovirus Vector Containing Chimeric Type 5 and 35 Fiber Protein(Biopharmacy)
- PHOSPHORYLATION OF RAT BASOPHILIC LEUKEMIA (RBL-2H3) CELL MEMBRANE PROTEINS IN RESPONSE TO VARIOUS STIMULI
- Large Grain Growth in Cu(In, Ga)Se_2 Thin Films with Band Gap of around 1.4 eV by Thermal Crystallization in Saturated Se Vapors
- Enzymes of purine catabolism in soybean plants
- Inhibitory Effect of Acetylshikonin on the Activation of NADPH Oxidase in Polymorphonuclear Leukocytes in Both Whole Cell and Cell-Free Systems
- Possible Involvement of Optimally Phosphorylated L-Plastin in Activation of Superoxide-Generating NADPH Oxidase
- Participation of Cofilin in Opsonized Zymosan-Triggered Activation of Neutrophil-Like HL-60 Cells through Rapid Dephosphorylation and Translocation to Plasma Membranes^1
- FLUCTUATION OF ENZYME ACTIVITIES RELATED TO NITROGEN FIXATION, C6/Cl RATIO, AND NICOTINAMIDE NUCLEOTIDE CONTENTS DURING SOYBEAN PLANT DEVELOPMENT
- Ion Cyclotron Heating by a Transversely Modulated Electron Beam-Plasma Interaction
- Asymmetric Synthesis of Optically Active Lactones from Cyclic Acid Anhydrides Using Lipase in Organic Solvents(Organic Chemistry)
- Levels of spinorphin in cerebrospinal fluid derived from patients with pain increase with decreasing dipeptidyl peptidase III
- A-36. Fatty Acid Composition of Brain Tumor Mitochondria
- Spinorphin, an Endogenous Inhibitor of Enkephalin-Degrading Enzymes, Potentiates Leu-Enkephalin-Induced Anti-allodynic and Antinociceptive Effects in Mice
- Hot Electron Production in a Beam-Produced Plasma
- A Study of Beam-Plasma Discharge. : I. Wave Characteristics
- COMPARISON OF ANTISPASMODIC EFFECT OF SYNTHETIC LYSOLECITHINS WITH VARIOUS FATTY ACID MOIETIES ON GUINEA PIG ILEUM
- Phorbor Myristate Acetate Induces NADPH Oxidase Activity of Cytochalasin B-Primed Neutrophils through the Protein Kinase C-Independent Pathway
- Mechanism of Testicular Damage by Phthalic Acid Esters (Proceedings of the 20th Symposium on Toxicology and Environmental Health)
- Possible Origin of Testicular Damage by Phthalic Acid Esters (Proceedings of the 22nd Symposium on Toxicology and Environmental Health)
- Carbon Metabolism in Relation to Cellular Organization of soybean Root Nodules and Respiration of Mitochondria Aided by Leghemoglobin
- Possible Origin of Testicular Atrophy Induced by 2,3,7,8-Tetrachlorodibenzo-p-dioxin (Proceedings of the 23rd Symposium on Toxicology and Environmental Health)
- Effect of Dexamethasone Pretreatment on the Dexamethasone-Depandent Induction of Tyrosine Aminotransferase Activity in Primary Cultured Rat Hepatocytes
- Genetic Polymorphisms of FCGRT Encoding FcRn in a Japanese Population and Their Functional Analysis
- Bioassay of Human Granulocyte Colony-Stimulating Factor Using Human Promyelocytic HL-60 Cells
- Effects of Calyculin A and FK506 on the O_2^- Generation of Rat Peritoneal Macrophages
- Simultaneous glycosylation analysis of human serum glycoproteins by high-performance liquid chromatography/tandem mass spectrometry
- The Hydroxyl Radical Formation System in Polymorphonuclear Leukocytes
- Participation of Cytosolic Protein Phosphatase in Regulation of NADPH Oxidase in Polymorphonuclear Leukocytes
- Time-Dependent Changes of Oxytocin Using 1H-NMR Coupled with Multivariate Analysis: a New Approach for Quality Evaluation of Protein/Peptide Biologic Drugs
- Ca and Mg Uptake by Soybean Plants at Different Phosphate Ion Concentrations
- Increase in the Formation and Nitrogen Fixation of Soybean Nodules by Vesicular-Arbuscular Mycorrhiza
- Changes in Crassulacean Acid Metabolism of Kalanchoe blossfeldiana by Different Nitrogen Sources
- The Significance of Glycosylation Analysis in Development of Biopharmaceuticals
- Incorporation of ^N into allantoin in nodulated soybean plants supplied with ^N_2
- Allantoin metabolism in soybean plants as influenced by grafts, a delayed inoculation with Rhizobium, and a late supply of nitrogen-compounds
- Effects of exogenous nitrogen-compounds on the concentrations of allantoin and various constituents in several organs of soybean plants
- Distribution and change in the contents of allantoin and allantoic acid in developing nodulating and non-nodulatng soybean plants
- Effects of Boron on Nodule Development and Symbiotic Nitrogen Fixation in Soybean Plants
- Effect of Heating Probe on Reactively Sputtered TiO_2 Film Growth
- Effects of Nitrate on Nucleotide Levels in Soybean Nodules
- Inhibition of Nitrogen Fixation in Soybean Plants Supplied with Nitrate I. Nitrite Accumulation and Formation of Nitrosylleghemoglobin in Nodules
- Sulfur abundance of asteroid 25143 Itokawa observed by X-ray fluorescence spectrometer onboard Hayabusa
- Promotion of Assimilation of Ammonium Ions by Simultaneous Application of Nitrate and Ammonium Ions in Radish Plants
- Formation of Nitrosylleghemoglobin in Nodules of Nitrate-Treated Cowpea and Pea Plants
- Inhibition of Nitrogen Fixation in Soybean Plants Supplied with Nitrate II. Accumulation and Properties of Nitrosylleghemoglobin in Nodules
- Effects of Different Nitrogen Sources on Glutamine Synthetase and Ferredoxin Dependent Glutamate Synthase Activities and on Free Amino Acid Composition in Radish Plants
- Inhibition of Nitrogen Fixation in Soybean Plants Supplied with Nitrate III. Kinetics of the Formation of Nitrosylleghemoglobin and of the Inhibition of Formation of Oxyleghemoglobin
- Measurement of Gas- and Particle-phase Organic Species in Diesel Exhaust Using Vacuum Ultraviolet Single Photon Ionization Time-of-flight Mass Spectrometry
- Measurement of Volatile Organic Compounds in Vehicle Exhaust Using Single-Photon Ionization Time-of-Flight Mass Spectrometry
- MOLECULAR BASIS OF THE DEBRISOQUINE 4-HYDROXYLASE DEFICIENCY IN DA RATS
- Foreword
- Possible Involvement of Optimally Phosphorylated L-Plastin in Activation of Superoxide-Generating NADPH Oxidase
- Participation of Cofilin in Opsonized Zymosan-Triggered Activation of Neutrophil-Like HL-60 Cells through Rapid Dephosphorylation and Translocation to Plasma Membranes.