Time-Dependent Changes of Oxytocin Using 1H-NMR Coupled with Multivariate Analysis: a New Approach for Quality Evaluation of Protein/Peptide Biologic Drugs
スポンサーリンク
概要
- 論文の詳細を見る
A new method that combines 1H-NMR and principal component analysis (PCA) was employed to obtain the quality evaluation of biopharmaceuticals, with regard to their quality, consistency, and differences in protein modification patterns. To assess the feasibility of the method, three 1H-NMR spectra of oxytocin (OXT) were collected every 7 d (at Day 0, 7 and 14), and time-dependent changes in the spectra were found by PCA of the 1H-NMR signals from 0.5—9.0 ppm, excluding the region around the water signal (4.6—5.0 ppm). Although the three OXT spectra seemed similar by simple visual inspection, time-dependent differences among the three spectra were clearly distinguished by a PCA scores plot. Peak changes indicating both OXT decomposition and the emergence of new OXT decomposition products within the timeframe of the experiment were also observed by a PCA loading plot. The results demonstrate that this method can evaluate the consistency of biopharmaceutical quality.
- 公益社団法人 日本薬学会の論文
著者
-
Kawasaki Nana
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
-
Okuda Haruhiro
Division Of Organic Chemistry National Institute Of Health Sciences
-
Yamaguchi Teruhide
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
-
Fukuhara Kiyoshi
Division Of Organic Chemistry
-
Ohno Akiko
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
-
Okuda Haruhiro
Division of Drugs, National Institute of Health Sciences
-
Fukuhara Kiyoshi
Division of Organic Chemistry, National Institute of Health Sciences
-
Kawasaki Nana
Division of Biological Chemistry & Biologicals, National Institute of Health Sciences
-
Yamaguchi Teruhide
Division of Biological Chemistry & Biologicals, National Institute of Health Sciences
関連論文
- Involvement of Electron Transfer in the Radical-scavenging Reaction of Resveratrol
- Activation of the Human Ah Receptor by Aza-Polycyclic Aromatic Hydrocarbons and Their Halogenated Derivatives
- Computational Study on Secondary Structure of Oligopeptides Containing α,α-Disubstituted α-Amino Acids
- Computational Study on Helical Structures of Oligopeptides Containing Chiral Cyclic α,α-Disubstituted α-Amino Acids
- Computational Study on Conformation of Oligopeptides Containing Chiral Cyclic α,α-Disubstituted α-Amino Acids
- Computational Study on Conformation of Oligopeptides Controlled by Side Chain Chiralities of α-Amino Acids
- Prediction of Helical Screw Sense of Oligopeptides Containing Chiral Amino Acids : Computational Study
- Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva
- EPR Study on Stable Magnesium Complexes of the Phenoxyl Radicals Derived from a Vitamin E Model and Its Deuterated Derivatives
- The Novel Compounds That Activate Farnesoid X Receptor : the Diversity of Their Effects on Gene Expression
- Antiviral Activity of 2-Amino-4, 4α-Dihydro-4α-7-Dimethyl-3H-Phenoxazine-3-One on Poliovirus
- Reduction Property and Mutagenicity of Newly Synthesized Nitroarenes as Environmental Mutagens (Proceedings of the 18th Symposium on Toxicology, and Environmental Health)
- Suppression of Proliferation of Poliovirus and Porcine Parvovirus by Novel Phenoxazines, 2-Amino-4,4α-dihydro-4α-7-dimethyl-3H-phenoxazine-3-one and 3-Amino-1,4α-dihydro-4α-8-dimethyl-2H-phenoxazine-2-one(Pharmacology)
- Unkeito, a traditional Kampo formula, exhibits a selective estrogen receptor modulator-like activity : Prevention of osteoporosis in ovariectomized mice
- A RELATIONSHIP BETWEEN ELECTROCHEMICAL REDUCTION POTENTIAL AND THE ENZYMATIC REDUCTION OF NITRATED NAPHTHALENES BY MICROSOMES OR CYTOSOL FROM RAT LIVER
- Annexin A3 Expression Increases in Hepatocytes and is Regulated by Hepatocyte Growth Factor in Rat Liver Regeneration
- Gene expression profiles of hepatotoxin-treated human hepatocytes can be used to cluster unknown compounds according to their mode of action(Toxicogenomics, Toxicoproteomics, Proceedings of the 32nd Annual Meeting)
- Prediction of Helical Screw Sense of Oligopeptides Containing Chiral α, α-Disubstituted Amino Acids
- Gene Expression Profiling of Human Mesenchymal Stem Cells for Identification of Novel Markers in Early- and Late-Stage Cell Culture
- TOXICITY STUDIES OF A SYNTHETIC ANTIOXIDANT, 2,2'-METHYLENEBIS (4-ETHYL-6-TERT-BUTYLPHENOL) IN RATS 2. UNCOUPLING EFFECT ON OXIDATIVE PHOSPHORYLATION OF LIVER MITOCHONDRIA
- SUGAR CHAIN STRUCTURE OF SPERM RECEPTOR HrVC70 ON THE VITELLINE COAT IN THE ASCIDIAN HALOCYNTHIA RORETZI(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Superoxide Anion Generation via Electron-Transfer Oxidation of Catechin Dianion by Molecular Oxygen in an Aprotic Medium
- 糖タンパク質の質量分析
- Present State of New Chiral Drug Development and Review in Japan
- Direct toxicity of ethane-1, 2-dimethanesulfonate to rat epididymis
- Hydroxylation of Nitrated Naphthalenes with KO_2/Crown Ether
- Possible Involvement of Optimally Phosphorylated L-Plastin in Activation of Superoxide-Generating NADPH Oxidase
- Computational Study on Helical Structure of α,α-Disubstituted Oligopeptides Containing Chiral α-Amino Acids
- Phorbor Myristate Acetate Induces NADPH Oxidase Activity of Cytochalasin B-Primed Neutrophils through the Protein Kinase C-Independent Pathway
- Enhancement of Hepatocyte Growth Factor-Induced Cell Scattering in N-Acetylglucosaminyltransferase III-transfected HepG2 Cells(Biochemistry/Molecular Biology)
- Effect of Dexamethasone Pretreatment on the Dexamethasone-Depandent Induction of Tyrosine Aminotransferase Activity in Primary Cultured Rat Hepatocytes
- Control of hemoglobin synthesis in erythroid differentiating K562 cells : II. Studies of iron mobilization in erythroid cells by high-performance liquid chromatography-electrochemical detection
- Genetic Polymorphisms of FCGRT Encoding FcRn in a Japanese Population and Their Functional Analysis
- Bioassay of Human Granulocyte Colony-Stimulating Factor Using Human Promyelocytic HL-60 Cells
- Simultaneous glycosylation analysis of human serum glycoproteins by high-performance liquid chromatography/tandem mass spectrometry
- Time-Dependent Changes of Oxytocin Using 1H-NMR Coupled with Multivariate Analysis: a New Approach for Quality Evaluation of Protein/Peptide Biologic Drugs
- The Significance of Glycosylation Analysis in Development of Biopharmaceuticals
- Design of Short α-Helical Peptides for Transcriptional Inhibitor of Nuclear Receptor
- Computational Study on Conformation of Oligopeptides Containing α,α-Disubstituted Amino Acids
- Conformational Studies of Diastereomeric -Leu-Aib- Peptides
- Design of Stapled Helical Peptides for Catalytic Enantioselective Epoxidation of α,β-Unsaturated Ketones
- Synthesis and Enhanced Radical Scavenging Activity of a Conformationally Constrained Epigallocatechin Analogue
- Rapid and Sensitive Method for Measuring the Plasma Concentration of Doxorubicin and Its Metabolites
- Kinetics and Mechanism for the Scavenging Reaction of the 2, 2-Diphenyl-1-picrylnydrazyl Radical by Synthetic Artepillin C Analogues
- Stilbene Analogs of Resveratrol Improve Insulin Resistance through Activation of AMPK
- A New Approach to Characterization of Insulin Derived from Different Species Using 1H-NMR Coupled with Multivariate Analysis
- Stilbene Analogs of Resveratrol Improve Insulin Resistance through Activation of AMPK
- Possible Involvement of Optimally Phosphorylated L-Plastin in Activation of Superoxide-Generating NADPH Oxidase