Enhancement of Hepatocyte Growth Factor-Induced Cell Scattering in N-Acetylglucosaminyltransferase III-transfected HepG2 Cells(Biochemistry/Molecular Biology)
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概要
- 論文の詳細を見る
N-Acetylglucosaminyltransferase III (GnT-HI), which catalyzes the synthesis of a bisecting GlcNAc residue of N-glycans, is thought to be involved in the function of glycoproteins such as growth factor receptors. We investigated the effects of the overexpression of GnT-III on the hepatocyte growth factor (HGF) receptor c-Met, a glycoprotein, in human hepatocarcinoma HepG2 cells. GnT-III activity was elevated about 250-fold in HepG2 cells stably transfected with the GnT-III gene, whereas no significant change in GnT-III activity was observed in mock transfectants. Cell scattering assay revealed that HGF-induced cell scattering was enhanced depending on the GnT-III activities in the GnT-III transfectants. Western blot analysis and E-PHA lectin blot analysis showed that the level of c-Met protein was the same in both transfectants; however, the bisecting GlcNAc residue on c-Met was detected only in the GnT-III transfectants. Although the peak level of c-Met phosphorylation was not different in both transfectants, the level of tyrosine phosphorylation of c-Met decreased more rapidly in the GnT-III transfectants than in the mock transfectants. Furthermore, HGF-induced extracellular-regulated kinase (ERK) phosphorylation was slightly higher in the GnT-III transfectants than in the mock transfectants. These results show that overexpression of GnT-III in HepG2 cells enhances HGF-induced cell scattering, which may result from, at least in part, enhancement of HGF-induced ERK phosphorylation.
- 公益社団法人日本薬学会の論文
- 2004-06-01
著者
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Kawasaki Nana
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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KAWANISHI Toru
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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HAYAKAWA Takao
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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HYUGA Masashi
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences of Biologica
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Kawanishi T
Division Of Drugs National Institute Of Health Science
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Kawanishi Toru
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Ohta Masato
Pharmaceutical Research Center Meiji Seika Kaisha Ltd.
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Hyuga Masashi
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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HARASHIMA Mizuho
Department of Nutrition and Physiology, Nihon University College of Bioresource Sciences
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NIIMI Shingo
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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Niimi S
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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KOBAYASI TADASHI
Kobe Pharmaceutical University
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Hayakawa Takao
Div. Of Biological Chemistry And Biologicals National Inst. Of Health Sciences Jpn
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Hayakawa T
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Hayakawa T
National Institute Of Health Sciences
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Hayakawa Takao
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Itoh Satsuki
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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Kawanishi Toru
Division Of Drugs National Institute Of Health Science
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Kawanishi Toru
国立医薬品食品衛生研究所
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Ohta Miyako
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Itoh Satsuki
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Hyuga Masashi
Department Of Nutrition And Physiology Nihon University College Of Bioresource Sciences
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HYUGA Sumiko
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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KAWASAKI Nana
National Institute of Health Sciences
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ITOH Satsuki
National Institute of Health Sciences
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Hyuga Sumiko
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Niimi Shingo
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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古林 隆司
宇部興産株式会社研究開発本部宇部研究所医薬研究部
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Kawasaki Nana
Division of Biological Chemistry & Biologicals, National Institute of Health Sciences
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Itoh Satsuki
Division of Biological Chemistry & Biologicals, National Institute of Health Sciences
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