A new device for the determination of microsomal cytochrome P-450 in renal tissue preparations from various species contaminated with mitochondria and hemoglobin.
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概要
- 論文の詳細を見る
Using a spectrophotometer connected to a microcomputer, the carbon monoxide difference spectrum of renal microsomal dithionitereduced cytochrome P-450 was measured avoiding the effects of the contaminating mitochondrial cytochromes and hemoglobin by subtracting the CO difference spectrum of a succinate-treated microsomal suspension from that of a dithionite-treated one. By this method, we quantitatively determined the microsomal cytochrome P-450 in kidneys from various species. The absorption peak of the renal microsomal cytochrome P-450 was about 452 nm in rats and about 450 nm in mice, hamsters, rabbits, guinea pigs, dogs, and pigs. Compared with the renal cytochrome P-450 contents in rats, the contents on a g tissue basis were greater in pigs, dogs, and mice and were about the same in hamsters and rabbits. The renal cytochrome P-450 contents in guinea pigs were less than those in rats.
- 公益社団法人 日本薬理学会の論文
著者
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Ohno Yasuo
Div. Of Pharmacology Biological Safty Research Center National Institute Of Hygienic Sciences
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Kawanishi Toru
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Omori Yoshihito
Division Of Pharmacology Biological Safety Research Center National Institute Of Hygienic Sciences:d
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Takahashi Atsushi
Division Of Electrical Electronic And Information Engineering Osaka University
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Ohno Yasuo
Division Of Internal Medicine And Thoracic Oncology National Cancer Center Hospital
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KASUYA Yutaka
Division of Pharmacology, Biological Safety Research Center, National Institute of Hygienic Sciences
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Takahashi Atsushi
Division Of Electrical Electronic And Information Engineering Graduate School Of Engineering Osaka University
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KAWANISHI Toru
Division of Pharmacology, Biological Safety Research Center, National Institute of Hygienic Sciences
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TAKAHASHI Atsushi
Division of Pharmacology, Biological Safety Research Center, National Institute of Hygienic Sciences
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OHNO Yasuo
Division of Pharmacology, Biological Safety Research Center, National Institute of Hygienic Sciences
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OMORI Yoshihito
Division of Pharmacology, Biological Safety Research Center, National Institute of Hygienic Sciences
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